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Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer: Association With Basal Markers and Functional Activities
- Source :
- Breast Cancer : Basic and Clinical Research, Breast Cancer: Basic and Clinical Research, Vol 12 (2018)
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- Basal-like breast cancer is an aggressive form of breast cancer with limited treatment options. The subgroup can be identified immunohistochemically, by lack of hormone receptor expression combined with expression of basal markers such as CK5/6 and/or epidermal growth factor receptor (EGFR). In vitro, several regulators of the actin cytoskeleton are essential for efficient invasion of basal-like breast cancer cell lines. Whether these proteins are expressed in vivo determines the applicability of these findings in clinical settings. The actin-regulating formin protein FHOD1 participates in invasion of the triple-negative breast cancer cell line MDA-MB-231. Here, we measure the expression of FHOD1 protein in clinical triple-negative breast cancers by using immunohistochemistry and further characterize the expression of another formin protein, INF2. We report that basal-like breast cancers frequently overexpress formin proteins FHOD1 and INF2. In cell studies using basal-like breast cancer cell lines, we show that knockdown of FHOD1 or INF2 interferes with very similar processes: maintenance of cell shape, migration, invasion, and proliferation. Inhibition of EGFR, PI3K, or mitogen-activated protein kinase activity does not alter the expression of FHOD1 and INF2 in these cell lines. We conclude that the experimental studies on these formins have implications in the clinical behavior of basal-like breast cancer.
- Subjects :
- DYNAMICS
0301 basic medicine
Cancer Research
CARCINOMA
MIGRATION
MDA-MB-231
3122 Cancers
macromolecular substances
lcsh:RC254-282
Formin
03 medical and health sciences
0302 clinical medicine
Breast cancer
medicine
PRIMARY THERAPY
Epidermal growth factor receptor
Protein kinase A
skin and connective tissue diseases
PI3K/AKT/mTOR pathway
Triple-negative breast cancer
GENE-EXPRESSION
Original Research
Gene knockdown
ACTIN-FILAMENT
biology
BT-549
INTERNATIONAL EXPERT CONSENSUS
cytoskeleton
ta3122
medicine.disease
Actin cytoskeleton
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
INF2
3. Good health
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Formins
CELLS
biology.protein
Cancer research
triple-negative breast cancer
CLINICAL-IMPLICATIONS
HIGHLIGHTS
FHOD1
basal-like breast cancer
actin
Subjects
Details
- Language :
- English
- ISSN :
- 11782234
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Breast Cancer : Basic and Clinical Research
- Accession number :
- edsair.doi.dedup.....c880cbdd77b57f2705227cc9341da561