Back to Search
Start Over
Olfactory Receptor OR7A17 Expression Correlates with All-Trans Retinoic Acid (ATRA)-Induced Suppression of Proliferation in Human Keratinocyte Cells
- Source :
- International Journal of Molecular Sciences; Volume 22; Issue 22; Pages: 12304, International Journal of Molecular Sciences, Vol 22, Iss 12304, p 12304 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Olfactory receptors (ORs), which belong to the G-protein-coupled receptor family, have been widely studied as ectopically expressed receptors in various human tissues, including the skin. However, the physiological functions of only a few OR types have been elucidated in skin cells. All-trans retinoic acid (ATRA) is a well-known medication for various skin diseases. However, many studies have shown that ATRA can have adverse effects, resulting from the suppression of cell proliferation. Here, we investigated the involvement of OR7A17 in the ATRA-induced suppression of human keratinocyte (HaCaT) proliferation. We demonstrated that OR7A17 is expressed in HaCaT keratinocytes, and its expression was downregulated by ATRA. The ATRA-induced downregulation of OR7A17 was attenuated via RAR α or RAR γ antagonist treatment, indicating that the effects of ATRA on OR7A17 expression were mediated through nuclear retinoic acid receptor signaling. Moreover, we found that the overexpression of OR7A17 induced the proliferation of HaCaT cells while counteracting the antiproliferative effect of ATRA. Mechanistically, OR7A17 overexpression reversed the ATRA-induced attenuation of Ca2+ entry. Our findings indicated that ATRA suppresses cell proliferation through the downregulation of OR7A17 via RAR α- and γ-mediated retinoid signaling. Taken together, OR7A17 is a potential therapeutic target for ameliorating the anti-proliferative effects of ATRA.
- Subjects :
- QH301-705.5
medicine.drug_class
olfactory receptor (OR)
Retinoic acid
Catalysis
Inorganic Chemistry
chemistry.chemical_compound
Downregulation and upregulation
medicine
Retinoid
Biology (General)
Physical and Theoretical Chemistry
Receptor
QD1-999
Molecular Biology
neoplasms
Spectroscopy
Olfactory receptor
Cell growth
Chemistry
organic chemicals
Organic Chemistry
all-trans retinoic acid (ATRA)
General Medicine
cell proliferation
calcium influx
G-protein-coupled receptor (GPCR)
overexpression
biological factors
Computer Science Applications
HaCaT
Retinoic acid receptor
medicine.anatomical_structure
Cancer research
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 22; Issue 22; Pages: 12304
- Accession number :
- edsair.doi.dedup.....c87c2e83a2f768f3eb357eb68fb15317
- Full Text :
- https://doi.org/10.3390/ijms222212304