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DNA binding, prominent photonuclease activity and antibacterial PDT of cobalt(II) complexes of phenanthroline based photosensitizers
- Source :
- Nucleosides, Nucleotides & Nucleic Acids. 37:546-562
- Publication Year :
- 2018
- Publisher :
- Informa UK Limited, 2018.
-
Abstract
- The chemistry of Co(II) complexes showing efficient light induced DNA cleavage activity, binding propensity to calf thymus DNA and antibacterial PDT is summarized in this article. Complexes of formulation [Co(mqt)(B)2]ClO4 1–3 where mqt is 4-methylquinoline-2-thiol and B is N,N-donor heterocyclic base, viz. 1,10-phenanthroline (phen 1), dipyrido[3,2-d:2′,3′-f]quinoxaline (dpq 2) and dipyrido[3,2-a:2′,3′-c]phenazine (dppz 3) have been prepared and characterized. The DNA-binding behaviors of these three complexes were explored by absorption spectra, viscosity measurements and thermal denaturation studies. The DNA binding constants for complexes 1, 2 and 3 were determined to be 1.6 × 103 M−1, 1.1 × 104 M−1 and 6.4 × 104 M−1 respectively. The experimental results suggest that these complexes interact with DNA through groove binding mode. The complexes show significant photocleavage of supercoiled (SC) DNA proceeds via a type-II process forming singlet oxygen as the reactive species. Antimicrobial photodynamic therapy was studied using photodynamic antimicrobial chemotherapy (PACT) assay against E. coli and all complexes exhibited significant reduction in bacterial growth on photoirradiation.
- Subjects :
- Magnetic Resonance Spectroscopy
Absorption spectroscopy
Stereochemistry
Phenanthroline
Phenazine
Drug Evaluation, Preclinical
chemistry.chemical_element
Phot
010402 general chemistry
01 natural sciences
Biochemistry
chemistry.chemical_compound
Quinoxaline
Quinoxalines
Escherichia coli
Organometallic Compounds
Genetics
Binding Sites
Photosensitizing Agents
Viscosity
010405 organic chemistry
Singlet oxygen
Cobalt
DNA
General Medicine
Anti-Bacterial Agents
0104 chemical sciences
Photochemotherapy
chemistry
Quinolines
Molecular Medicine
Triazenes
Phenanthrolines
Subjects
Details
- ISSN :
- 15322335 and 15257770
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Nucleosides, Nucleotides & Nucleic Acids
- Accession number :
- edsair.doi.dedup.....c8235b02817474cc55059c54b867c82a