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Mouse tetherin enhances moloney murine leukemia virus-induced syncytium formation
- Source :
- Acta virologica. 60(4)
- Publication Year :
- 2016
-
Abstract
- Tetherin (also referred to as BST-2 or CD317) is an antiviral cellular restriction factor that inhibits the release of many enveloped viruses. It is a 30-36 kDa type II transmembrane protein, expression of which is induced by type I interferon. Mouse tetherin inhibits nascent cell-free particle release. However, it is unclear whether mouse tetherin restricts cell-to-cell spread of moloney murine leukemia virus (Mo-MLV) or whether is the mouse tetherin involved in syncytium formation. To examine cell-to-cell spread and syncytium formation of Mo-MLV in the presence or absence of mouse tetherin, R peptide (the cytoplasmic tail of the transmembrane protein (TM); 16 amino acids) truncated Env expressing vector was constructed. It contained enhanced green fluorescent protein (EGFP) in the proline rich region (PRR) of Env. This R(-)Env full-length molecular clone could rule out virus-cell transmission due to the slightly reduced R(-)Env protein incorporation into the viral particles. When NIH3T3 cells stably expressing mouse tetherin were transfected with R(-)Env full-length molecular clone, syncytium formation was significantly enhanced in the tetherin-expressing cells. These data suggest that tetherin-mediated retention of R-defective virions on the cell surface could enhance syncytium formation. In addition, we found that the R(-)Env full-length molecular clone containing EGFP in the PRR of Env to be a useful tool allowing fast and convenient detection of syncytia by fluorescence microscopy.
- Subjects :
- 0301 basic medicine
viruses
Clone (cell biology)
Giant Cells
Green fluorescent protein
Rodent Diseases
03 medical and health sciences
Mice
Viral envelope
Interferon
Antigens, CD
Virology
Murine leukemia virus
medicine
Animals
Virus Release
Syncytium
Membrane Glycoproteins
biology
Chemistry
Virion
virus diseases
General Medicine
biology.organism_classification
Transmembrane protein
Cell biology
030104 developmental biology
Infectious Diseases
Tetherin
NIH 3T3 Cells
Moloney murine leukemia virus
medicine.drug
Retroviridae Infections
Subjects
Details
- ISSN :
- 0001723X
- Volume :
- 60
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Acta virologica
- Accession number :
- edsair.doi.dedup.....c81df3e8f64e356c1ad2704766117bb8