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Detection and cloning of a protein recognized by anti-human prostate-specific antigen (PSA) antibody in the rat ventral prostate
- Source :
- Japanese Journal of Cancer Research : Gann
- Publication Year :
- 2001
-
Abstract
- Prostate-specific antigen (PSA), a 33 kDa glycoprotein produced in the epithelium of the human prostate, has become established as a useful tumor marker for prostate cancer in man. Since reports of homologous proteins in animals other than primates have been lacking, the present investigation was carried out to identify any PSA-like protein in rats. Immunoblot analysis using a specific monoclonal anti-human PSA antibody detected a 32 kDa immunoreactive protein in the ventral lobe of the rat prostate, but not in other lobes or in other tissues. Positive immunostaining was observed only for the luminal surface of the glandular epithelium and the intraductal fluid in the ventral prostate. Sequence analysis of a cDNA for the rat PSA-like protein, cloned by immunoscreening of an expression cDNA library prepared from the ventral lobe, revealed identity to the rat submaxillary gland S3 kallikrein. Human PSA also belongs to the kallikrein family. Thus, this protein produced in the rat ventral prostate was suggested to be a possible counterpart of human PSA.
- Subjects :
- Male
Cancer Research
DNA, Complementary
Immunoblotting
Molecular Sequence Data
Biology
urologic and male genital diseases
Article
Immunoenzyme Techniques
Prostate cancer
Mice
Antigen
Prostateāspecific antigen
Immunoscreening
medicine
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Ventral prostate
PSA Antibody
Sequence Homology, Amino Acid
cDNA library
Prostate
Antibodies, Monoclonal
Kallikrein
Prostate-Specific Antigen
medicine.disease
Molecular biology
Rats, Inbred F344
Rats
Prostate-specific antigen
Oncology
Rat
Electrophoresis, Polyacrylamide Gel
Kallikreins
Rabbits
Immunostaining
Subjects
Details
- ISSN :
- 09105050
- Volume :
- 92
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Japanese journal of cancer research : Gann
- Accession number :
- edsair.doi.dedup.....c81b9e4db045762e991b8779dad1eb79