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Effect of Shenqin biochemical extract on hypoxia-inducible factor-1α expression in ultraviolet B-irradiated HaCaT cells
- Source :
- Genetics and molecular research : GMR. 16(2)
- Publication Year :
- 2017
-
Abstract
- Hypoxia-inducible factor-1α (HIF-1α) is considered the main transcriptional regulator of the hypoxia-specific cellular and developmental response. This study was performed to investigate the effect of Shenqin biochemical extract (SQBE) on HIF-1α expression in ultraviolet B (UVB)-irradiated HaCaT cells and the possible action mechanisms of SQBE against UVB-induced skin cancer. HaCaT cells in logarithmic growth phase were seeded in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum, and conventionally cultured at 37°C with 5% CO2. Cells were divided into control group (administered the same amounts of dimethyl sulfoxide), SQBE1 group (12.5 μg/mL SQBE), SQBE2 group (25.0 μg/mL SQBE), and SQBE3 group (50.0 μg/mL SQBE). Four hours post administration, the control and treatment groups were irradiated with UVB (0, 20, 40, and 60 mJ/cm2). After 24 h, cell survival rate was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Expression levels of HIF-1α mRNA and protein were detected by polymerase chain reaction and western blotting, respectively. SQBE-treated, UVB-irradiated cells had improved survival rates. This increase was most significant in SQBE3 group (P < 0.01), which also had effectively reduced expression of intracellular HIF-1α mRNA and protein. Hence, SQBE had a protective effect on UVB-irradiated HaCaT cells and inhibited the UVB irradiation-induced expression of HIF-1α. This indicates that SQBE could prevent the occurrence of UVB radiation-induced skin cancer.
- Subjects :
- Keratinocytes
Messenger RNA
integumentary system
Dimethyl sulfoxide
Scutellaria
Ultraviolet Rays
Panax
General Medicine
Biology
Hypoxia-Inducible Factor 1, alpha Subunit
Molecular biology
Blot
chemistry.chemical_compound
HaCaT
chemistry
Hypoxia-inducible factors
Cell Line, Tumor
Immunology
Genetics
Transcriptional regulation
Humans
Molecular Biology
Fetal bovine serum
Intracellular
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 16765680
- Volume :
- 16
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Genetics and molecular research : GMR
- Accession number :
- edsair.doi.dedup.....c81107f9dbffc6a0098f5859867bc872