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Otefin, a Nuclear Membrane Protein, Determines the Fate of Germline Stem Cells in Drosophila via Interaction with Smad Complexes
- Source :
- Developmental Cell. 14:494-506
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Nuclear envelope proteins play important roles in chromatin organization, gene regulation, and signal transduction; however, the physiological role of these proteins remains elusive. We found that otefin (ote), which encodes a nuclear lamin-binding protein [corrected], is essential for germline stem cell (GSC) maintenance. We show that Ote, as an intrinsic factor, is both necessary and sufficient to regulate GSC fate. Furthermore, we demonstrate that ote is required for the Dpp/BMP signaling pathway to silence bam transcription. By structure-function analysis, we demonstrate that the nuclear membrane localization of Ote is essential for its role in GSC maintenance. Finally, we show that Ote physically interacts with Medea/Smad4 at the bam silencer element to regulate GSC fate. Thus, we demonstrate that specific nuclear membrane components mediate signal-dependent transcriptional effects to control stem cell behavior.
- Subjects :
- animal structures
Nuclear Envelope
Recombinant Fusion Proteins
SMAD
Regulatory Sequences, Nucleic Acid
Biology
General Biochemistry, Genetics and Molecular Biology
medicine
Animals
Drosophila Proteins
Gene Silencing
Nuclear membrane
Nuclear protein
BMP signaling pathway
Molecular Biology
Cells, Cultured
Smad4 Protein
Genetics
Stem Cells
Ovary
Membrane Proteins
Nuclear Proteins
Cell Biology
STEMCELL
Cell biology
Chromatin
Drosophila melanogaster
Germ Cells
medicine.anatomical_structure
Membrane protein
SIGNALING
Bone Morphogenetic Proteins
Mutation
embryonic structures
Nuclear lamina
Female
RNA Interference
Signal transduction
Signal Transduction
Developmental Biology
Subjects
Details
- ISSN :
- 15345807
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Developmental Cell
- Accession number :
- edsair.doi.dedup.....c801a2c66e75ba979a50ad0a179c31d3