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Sustained Exposure of Substance P Causes Tendinopathy
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 22, International Journal of Molecular Sciences, Vol 21, Iss 8633, p 8633 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- Recently, neuromediators such as substance P (SP) have been found to be important factors in tendon homeostasis. Some studies have found SP to be the cause of inflammation and tendinopathy, whereas others have determined it to be a critical component of tendon healing. As demonstrated by these conflicting findings, the effects of SP on tendinopathy remain unclear. In this study, we hypothesized that the duration of SP exposure determines its effect on the tendons, with repetitive long-term exposure leading to the development of tendinopathy. First, we verified the changes in gene and protein expression using in vitro tenocytes with 10-day exposure to SP. SP and SP + Run groups were injected with SP in their Achilles tendon every other day for 14 days. Achilles tendons were then harvested for biomechanical testing and histological processing. Notably, tendinopathic changes with decreased tensile strength, as observed in the Positive Control, were observed in the Achilles in the SP group compared to the Negative Control. Subsequent histological analysis, including Alcian blue staining, also revealed alterations in the Achilles tendon, which were generally consistent with the findings of tendinopathy in SP and SP + Run groups. Immunohistochemical analysis revealed increased expression of SP in the SP group, similar to the Positive Control. In general, the SP + Run group showed worse tendinopathic changes. These results suggest that sustained exposure to SP may be involved in the development of tendinopathy. Future research on inhibiting SP is warranted to target SP in the treatment of tendinopathy and may be beneficial to patients with tendinopathy.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
tendon
substance P
Substance P
Inflammation
Achilles Tendon
Catalysis
Article
lcsh:Chemistry
Inorganic Chemistry
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
degenerative
Animals
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Cells, Cultured
Achilles tendon
business.industry
Organic Chemistry
030229 sport sciences
General Medicine
medicine.disease
tenocyte
In vitro
Computer Science Applications
Tendon
Rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Tendinopathy
Immunohistochemistry
medicine.symptom
business
Homeostasis
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....c7fe454c505748bdc76f8f1930ac3187