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Oldhamianoside II, a New Triterpenoid Saponin, Prevents Tumor Growth via Inducing Cell Apoptosis and Inhibiting Angiogenesis
- Source :
- Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics. 20:369-376
- Publication Year :
- 2013
- Publisher :
- Computers, Materials and Continua (Tech Science Press), 2013.
-
Abstract
- Oldhamianoside II is a new triterpenoid saponin that was isolated from the roots of Gypsophila oldhamiana. The present study aims to investigate the potential inhibitory activity of oldhamianoside II on tumor growth using an S180 tumor implantation mouse model. Oldhamianoside II at doses of 5.0 and 10.0 mg/kg was given with intraperitoneal injection for 10 days following subcutaneous inoculation of S180 tumor cells in anterior flank of mice. The tumor growth, the cell apoptosis, the microvessel density (MVD) in S180 tumors, the tumor cell viability, the tubular formation in vitro, and migration of tumor cells were examined. The expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and cyclooxygenase-2 (COX-2) was determined to analyze the associated mechanisms. The results showed that oldhamianoside II potently inhibited tumor cell viability in vitro. In addition, oldhamianoside II delayed tumor growth in anterior flank, induced S180 cell apoptosis, and reduced the MVD. Oldhamianoside II was also demonstrated to decrease the number of tubular structure and vessel formation in HUVEC cultures and chick embryo chorioallantoic membrane (CAM) model, respectively. Further study indicated that oldhamianoside II reduced the expression of VEGF, bFGF, and COX-2 in tumor sections. Moreover, oldhamianoside II inhibited the activity of migration and penetration to Matrigel of SGC7901 tumor cells in scratch wound and transwell chamber. In conclusion, our work defines oldhamianoside II, a new triterpenoid saponin, as a novel compound that can effectively inhibit S180 tumor growth, induce tumor cell apoptosis, prevent tumor angiogenesis, and inhibit cancer cell migration, suggesting that oldhamianoside II is a potential drug candidate for the treatment of cancer and for the prevention of metastasis.
- Subjects :
- Vascular Endothelial Growth Factor A
Cancer Research
Angiogenesis
Basic fibroblast growth factor
Angiogenesis Inhibitors
Antineoplastic Agents
Apoptosis
Biology
Metastasis
Mice
chemistry.chemical_compound
Cell Movement
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Matrigel
General Medicine
Saponins
medicine.disease
Triterpenes
In vitro
Vascular endothelial growth factor
Chorioallantoic membrane
Oncology
chemistry
Immunology
Cancer research
Female
Fibroblast Growth Factor 2
Subjects
Details
- ISSN :
- 09650407
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics
- Accession number :
- edsair.doi.dedup.....c7f197fa34791bd70deb16b0a8b3606a