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In vitro tubulogenesis of Madin–Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration
- Source :
- Journal of Theoretical Biology. 435:110-115
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- In vitro tubulogenesis has been employed as an experimental model system to study tissue morphogenesis of internal organs. It has been previously shown that Madin-Darby canine kidney (MDCK) cells form tubes in the presence of hepatocyte growth factor in 3D cultures. Although these cells are expected to form tube structures in some microenvironments independent of chemical stimulation, little is known about the cellular mechanisms in organizing such an anisotropic multicellular structure. Here, we report 3D culture conditions that induce MDCK tubulogenesis without growth factor stimulation. We found that the cells spontaneously form elongated tube structures through aggregation processes in a specific range of both constituent cell number and scaffold gel concentration, while they form spherical aggregates in other conditions. We then examined cellular activities affecting tubulogenesis and showed that cell proliferation is not required for the tube elongation. Furthermore, we revealed that cells in the tube tips generate traction forces and pull the surrounding scaffold gel to migrate, resulting in the tube elongation. Our results suggest that the constituent cells during the aggregation process interact each other via mechanical forces transmitted in the scaffold gel, leading to the spontaneous tube formation.
- Subjects :
- 0301 basic medicine
Statistics and Probability
Scaffold
medicine.medical_treatment
Morphogenesis
Cell Count
Biology
General Biochemistry, Genetics and Molecular Biology
Madin Darby Canine Kidney Cells
03 medical and health sciences
Dogs
Spheroids, Cellular
medicine
Animals
Cell Aggregation
Tube formation
General Immunology and Microbiology
Cell growth
Applied Mathematics
Growth factor
Spheroid
General Medicine
In vitro
Biomechanical Phenomena
Cell biology
Kidney Tubules
030104 developmental biology
Modeling and Simulation
Hepatocyte growth factor
General Agricultural and Biological Sciences
Gels
medicine.drug
Subjects
Details
- ISSN :
- 00225193
- Volume :
- 435
- Database :
- OpenAIRE
- Journal :
- Journal of Theoretical Biology
- Accession number :
- edsair.doi.dedup.....c7e147c9e172707e0e0288c261162617
- Full Text :
- https://doi.org/10.1016/j.jtbi.2017.09.009