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Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte
- Source :
- Fungal Biology. 125:39-48
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- This study was undertaken to explore alternative applications of the widely known entomopathogenic/endophytic fungus, Beauveria bassiana, besides its sole use as a biocontrol agent. B. bassiana SAN01, was investigated for the production of two glycoside hydrolases, xylanase and endoglucanase under submerged conditions. Among the different biomass tested, wheat bran provided the best results for both xylanase and endoglucanase, and their production levels were further enhanced using response surface methodology. Under optimised conditions, heightened yields of 1061 U/ml and 23.03 U/ml were observed for xylanase and endoglucanase, respectively, which were 3.44 and 1.35 folds higher than their initial yields. These are the highest ever production levels reported for xylanase and endoglucanase from any B. bassiana strain or any known entomopathogenic fungi. Furthermore, the efficacy of xylanase/endoglucanase cocktail in the saccharification of sugarcane bagasse was evaluated. The highest amount of reducing sugar released from the pretreated biomass by the action of the crude Beauveria enzyme cocktail was recorded at 30°C after 8 h incubation. The significant activities of the hydrolytic enzymes recorded with B. bassiana in this study thus present promising avenues for the use of the entomopathogen as a new source of industrial enzymes and by extension, other biotechnological applications.
- Subjects :
- 0106 biological sciences
Beauveria bassiana
Cellulase
Biology
01 natural sciences
03 medical and health sciences
Endophytes
Genetics
Glycoside hydrolase
Food science
Beauveria
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Bran
fungi
biology.organism_classification
Saccharum
Reducing sugar
Xylosidases
Infectious Diseases
chemistry
biology.protein
Xylanase
Bagasse
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18786146
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Fungal Biology
- Accession number :
- edsair.doi.dedup.....c7e03f1f5d2bd9fadb771ccc2f01637b