Phylogenetic Analysis and Karyotype Evolution in Two Species of Core Gruiformes: Aramides cajaneus and Psophia viridis

This research was partially funded by a grant to EHCO from CNPq (307382/2019-2) and to MAFS from the Wellcome Trust in support of the Cambridge Resource Centre for comparative genomics Federal University of Par?. Post-Graduation Program in Genetics and Molecular Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecido e Citogen?tica. Ananindeua, PA, Brasil / Cambridge Resource Centre for Comparative Genomics. Cambridge, UK. Cambridge Resource Centre for Comparative Genomics. Cambridge, UK / Federal University of Rio Grande do Sul. Pos-Graduation Program in Genetics and Molecular Biology. Porto Alegre, RS, Brazil. Cambridge Resource Centre for Comparative Genomics. Cambridge, UK. Cambridge Resource Centre for Comparative Genomics. Cambridge, UK. Cambridge Resource Centre for Comparative Genomics. Cambridge, UK. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecido e Citogen?tica. Ananindeua, PA, Brasil / Federal University of Par?. Faculty of Natural Sciences. Institute of Exact and Natural Sciences. Bel?m, PA, Brazil. Abstract: Gruiformes is a group with phylogenetic issues. Recent studies based on mitochondrial and genomic DNA have proposed the existence of a core Gruiformes, consisting of five families: Heliornithidae, Aramidae, Gruidae, Psophiidae and Rallidae. Karyotype studies on these species are still scarce, either by conventional staining or molecular cytogenetics. Due to this, this study aimed to analyze the karyotype of two species (Aramides cajaneus and Psophia viridis) belonging to families Rallidae and Psopiidae, respectively, by comparative chromosome painting. The results show that some chromosome rearrangements in this group have different origins, such as the association of GGA5/GGA7 in A. cajaneus, as well as the fission of GGA4p and association GGA6/GGA7, which place P. viridis close to Fulica atra and Gallinula chloropus. In addition, we conclude that the common ancestor of the core Gruiformes maintained the original syntenic groups found in the putative avian ancestral karyotype