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Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation
- Source :
- Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-16 (2017), Stem Cell Research & Therapy
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significance in tissue regeneration and in hemopoietic niche regulation. On the contrary, information regarding the MPC angiogenic process is still incomplete and requires further clarification. In particular, genuine MPC angiogenic potential should be confirmed in-vivo. Methods In the present article, markers and functions associated with angiogenic cells have been dissected. MPCs freshly isolated from human bone marrow have been induced to differentiate into exponentially growing MSCs (P2-MSCs). Cells have been characterized and angiogenesis-related gene expression was evaluated before and after mesengenic differentiation. Moreover, angiogenic potential has been tested by in-vitro and in-vivo functional assays. Results MPCs showed a distinctive gene expression profile, acetylated-low density lipoprotein uptake, and transendothelial migration capacity. However, mature endothelial markers and functions of endothelial cells, including the ability to form new capillaries, were absent, thus suggesting MPCs to be very immature endothelial progenitors. MPCs showed marked 3D spheroid sprouting activating the related molecular machinery, a clear in-vitro indication of early angiogenesis. Indeed, MPCs applied to chicken chorioallantoic membrane induced and participated in neovessel formation. All of these features were lost in mesengenic terminally differentiated P2-MSCs, showing definite separation of the two differentiation lineages. Conclusion Our results confirm the bona-fide angiogenic potential of MPCs and suggest that the high variability reported for MSC cultures, responsible for the controversies regarding MSC angiogenic potential, could be correlated to variable percentages of co-isolated MPCs in the different culture conditions so far used. Electronic supplementary material The online version of this article (doi:10.1186/s13287-017-0562-x) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Male
Pathology
Angiogenesis
Cellular differentiation
TEM1
Mesenchymal stromal cells
Medicine (miscellaneous)
Mesangiogenic progenitor cells
Genetics and Molecular Biology (miscellaneous)
Biochemistry
Chorioallantoic membrane assay
Nestin
0302 clinical medicine
DLL-4
Adipocytes
lcsh:QD415-436
Cells, Cultured
lcsh:R5-920
Cultured
Cell Differentiation
Middle Aged
Cell biology
Haematopoiesis
Chorioallantoic membrane
Adult Stem Cells
030220 oncology & carcinogenesis
Molecular Medicine
Female
Stem cell
lcsh:Medicine (General)
Adult stem cell
medicine.medical_specialty
Bone marrow-derived cells
Sprouting
Human Umbilical Vein Endothelial Cells
Humans
Mesenchymal Stem Cells
Neovascularization, Physiologic
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Cell Biology
Cells
Biology
lcsh:Biochemistry
03 medical and health sciences
medicine
Progenitor cell
Physiologic
Neovascularization
Research
Mesenchymal stem cell
030104 developmental biology
Subjects
Details
- Language :
- English
- ISSN :
- 17576512
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research & Therapy
- Accession number :
- edsair.doi.dedup.....c7ad7c2de08a92cdcd34731cd357da6a
- Full Text :
- https://doi.org/10.1186/s13287-017-0562-x