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Rapid classification of chromoblastomycosis agents genera by infrared spectroscopy and chemometrics supervised by sequencing of rDNA regions
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 254
- Publication Year :
- 2020
-
Abstract
- Chromoblastomycosis (CBM) is a skin and subcutaneous infection caused by species of seven fungal genera. Identification of CBM species is performed by DNA sequencing of one or more genes, which becomes a time-consuming work. Fourier Transform Infrared Spectroscopy (FTIR) has been used for the identification of other microorganisms, however, only one CBM genus was evaluated by FTIR analysis to date. Therefore, the study is aimed to differentiate the CBM agents for identification at genera level using FTIR supervised by Internal Transcribed Spacer (ITS) rDNA region. Seventy-seven isolates of the main five CBM genera were prepared for Attenuated Total Reflection FTIR (ATR-FTIR) with a new methodology using slices of dry fungus in glass fixing-modeling proposed in this study. The algorithm Hierarchical Cluster Analysis (HCA) was used to analyze the differences and similarities between species through the spectra. Orthogonal Partial Least Square Discriminant Analysis (OPLS-DA) allowed to correctly classify all samples of five CBM genera. The ATR-FTIR/OPLS-DA models highlighted important contributions of regions attributed to NH and OH stretching, amide I of proteins, polysaccharides bands and fingerprint region for the complete differentiation of the genera investigated. Thus, FTIR can be a fast and inexpensive alternative for identification of CBM agents.
- Subjects :
- Infrared spectroscopy
02 engineering and technology
Computational biology
010402 general chemistry
01 natural sciences
DNA, Ribosomal
DNA sequencing
Analytical Chemistry
Chemometrics
stomatognathic system
Genus
Spectroscopy, Fourier Transform Infrared
medicine
Humans
Internal transcribed spacer
Fourier transform infrared spectroscopy
Least-Squares Analysis
Instrumentation
Spectroscopy
Chromoblastomycosis
Chemistry
Discriminant Analysis
021001 nanoscience & nanotechnology
medicine.disease
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Attenuated total reflection
0210 nano-technology
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 254
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....c794fbc1d5810c1482479550f392d0fb