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CD36 Is Upregulated in Mice with Periodontitis and Metabolic Syndrome and Involved in Macrophage Gene Upregulation by Palmitate
- Publication Year :
- 2017
-
Abstract
- Background We reported that high-fat diet (HFD)-induced metabolic syndrome (MetS) exacerbates lipopolysaccharide (LPS)-stimulated periodontitis and palmitate, the major saturated fatty acid in the HFD, amplified LPS-stimulated gene expression in vitro. Since CD36 is a major receptor for fatty acids, we investigated periodontal CD36 expression in mice with periodontitis and MetS, and the role of CD36 in inflammatory genes in macrophages stimulated by palmitate. Methods MetS and periodontitis were induced in mice by HFD and periodontal injection of LPS, respectively. The periodontal CD36 expression and its relationship with alveolar bone loss were studied using immunohistochemistry, real-time PCR, and correlation analysis. The role of CD36 in upregulation of inflammatory mediators by LPS and palmitate in macrophages was assessed using pharmacological inhibitor and small interfering RNA. Results Periodontal CD36 expression was higher in mice with both MetS and periodontitis than that in mice with periodontitis or MetS alone and was correlated with osteoclastogenesis and alveolar bone loss. In vitro studies showed that CD36 expression in macrophages was upregulated by LPS and palmitate, and targeting CD36 attenuated palmitate-enhanced gene expression. Conclusion CD36 expression is upregulated in mice with periodontitis and MetS, and involved in gene expression in macrophages stimulated by palmitate and LPS. This article is protected by copyright. All rights reserved.
- Subjects :
- 0301 basic medicine
CD36 Antigens
Lipopolysaccharides
Male
medicine.medical_specialty
Small interfering RNA
Lipopolysaccharide
CD36
Alveolar Bone Loss
Palmitic Acid
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Downregulation and upregulation
Osteogenesis
Internal medicine
parasitic diseases
Gene expression
medicine
Gene silencing
Animals
Gene Silencing
Periodontitis
General Dentistry
Cells, Cultured
Metabolic Syndrome
biology
Macrophages
030206 dentistry
medicine.disease
Up-Regulation
030104 developmental biology
Endocrinology
Otorhinolaryngology
chemistry
Saturated fatty acid
biology.protein
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c775275b79408f5f96b190670fca0591