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Characterization of the Host Response to Pichinde Virus Infection in the Syrian Golden Hamster by Species-Specific Kinome Analysis*
- Publication Year :
- 2015
- Publisher :
- The American Society for Biochemistry and Molecular Biology, 2015.
-
Abstract
- The Syrian golden hamster has been increasingly used to study viral hemorrhagic fever (VHF) pathogenesis and countermeasure efficacy. As VHFs are a global health concern, well-characterized animal models are essential for both the development of therapeutics and vaccines as well as for increasing our understanding of the molecular events that underlie viral pathogenesis. However, the paucity of reagents or platforms that are available for studying hamsters at a molecular level limits the ability to extract biological information from this important animal model. As such, there is a need to develop platforms/technologies for characterizing host responses of hamsters at a molecular level. To this end, we developed hamster-specific kinome peptide arrays to characterize the molecular host response of the Syrian golden hamster. After validating the functionality of the arrays using immune agonists of defined signaling mechanisms (lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-α), we characterized the host response in a hamster model of VHF based on Pichinde virus (PICV(1)) infection by performing temporal kinome analysis of lung tissue. Our analysis revealed key roles for vascular endothelial growth factor (VEGF), interleukin (IL) responses, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling, and Toll-like receptor (TLR) signaling in the response to PICV infection. These findings were validated through phosphorylation-specific Western blot analysis. Overall, we have demonstrated that hamster-specific kinome arrays are a robust tool for characterizing the species-specific molecular host response in a VHF model. Further, our results provide key insights into the hamster host response to PICV infection and will inform future studies with high-consequence VHF pathogens.
- Subjects :
- Vascular Endothelial Growth Factor A
Proteome
Viral pathogenesis
Hamster
Biology
Biochemistry
Hemorrhagic Fever, American
Analytical Chemistry
Viral hemorrhagic fever
Immune system
Species Specificity
medicine
Animals
Kinome
Phosphorylation
Molecular Biology
Lung
Pichinde virus
Mesocricetus
Research
Interleukins
Toll-Like Receptors
NF-kappa B
medicine.disease
biology.organism_classification
Virology
Disease Models, Animal
Female
Signal transduction
Protein Kinases
Golden hamster
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c774e184cbb2b7e3b4b38c49b745e4bf