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The vitamin A family can significantly decrease the expression of ERĪ² of ERs positive breast cancer cells in the presence or absence of ER ligands and paclitaxel
- Source :
- Gynecological Endocrinology. 25:287-293
- Publication Year :
- 2009
- Publisher :
- Informa UK Limited, 2009.
-
Abstract
- Taxanes have high activity against breast cancer cells either as the single agent or in combination with other anticancer compounds. The aim of the study was to determine the effects of vitamin A compounds on the cytotoxic action of paclitaxel and on the expression of ERs in the MCF-7 breast cancer cells. Retinol and beta-carotene, but not retinoids, added to the culture exerted an effect on paclitaxel activity. However, only beta-carotene significantly reduced the percentage of proliferating cells (40.36% +/- 5.64, p < 0.01). We observed that vitamin A and its derivatives combined with paclitaxel and estradiol decreased the percentage of proliferating cells, but only in comparison to estradiol group, whereas retinol and lycopene administered together with paclitaxel and tamoxifen decrease significantly the percentage of proliferatin cells (36.85% +/- 4.71, p < 0.0001 and 37.22% +/- 1.59, p < 0.0001 respectively, compared with paclitaxel group). We have shown that paclitaxel increases the expression of ERalpha and ERbeta mRNA in MCF-7 line. The strongest effect of transcription inhibition ERalpha (2.5 times) and especially ERbeta (10 times) was observed after addition of 9-cis retinoic acid and paclitaxel. This data suggests a synergistic effect of the compounds on ERbeta down-regulation. Our results support the use of retinoid is treatment of ER positive breast cancer patients.
- Subjects :
- Vitamin
medicine.medical_specialty
Paclitaxel
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Retinoic acid
Estrogen receptor
Breast Neoplasms
Ligands
chemistry.chemical_compound
Lycopene
Endocrinology
Cell Line, Tumor
Internal medicine
medicine
Estrogen Receptor beta
Humans
RNA, Messenger
Retinoid
Vitamin A
Cell Proliferation
Estrogen Antagonists
Estrogen Receptor alpha
Retinol
Obstetrics and Gynecology
Vitamins
beta Carotene
Antineoplastic Agents, Phytogenic
Carotenoids
Tamoxifen
chemistry
MCF-7
Female
medicine.drug
Subjects
Details
- ISSN :
- 14730766 and 09513590
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Gynecological Endocrinology
- Accession number :
- edsair.doi.dedup.....c7709ca06e5abeae0346ce9688793278