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SP1 induced long non-coding RNA AGAP2-AS1 promotes cholangiocarcinoma proliferation via silencing of CDKN1A
- Source :
- Molecular Medicine, Vol 27, Iss 1, Pp 1-10 (2021), Molecular Medicine
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Background LncRNA can regulate gene at various levels such as apparent genetics, alternative splicing, and regulation of mRNA degradation. However, the molecular mechanism of LncRNA in cholangiocarcinoma is still unclear. This deserves further exploration. Methods We investigated the expression of AGAP2-AS1 in 32 CCA tissues and two CCA cell lines. We found a LncRNA AGAP2-AS1 which induced by SP1 has not been reported in CCA, and Knockdown and overexpression were used to investigate the biological role of AGAP2-AS1 in vitro. CHIP and RIP were performed to verify the putative targets of AGAP2-AS1. Results AGAP2-AS1 was significantly upregulated in CCA tumor tissues. SP1 induced AGAP2-AS1 plays an important role in tumorigenesis. AGAP2-AS1 knockdown significantly inhibited proliferation and caused apoptosis in CCA cells. In addition, we demonstrated that AGAP2-AS1 promotes the proliferation of CCA. Conclusions We conclude that the long non-coding RNA AGAP2-AS1 plays a role in promoting the proliferation of cholangiocarcinoma.
- Subjects :
- Male
0301 basic medicine
Sp1 Transcription Factor
Biology
medicine.disease_cause
Cholangiocarcinoma
lcsh:Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
AGAP2-AS1
Downregulation and upregulation
Cell Line, Tumor
parasitic diseases
Genetics
medicine
Animals
Humans
Gene silencing
lcsh:QD415-436
EZH2
CCA
Molecular Biology
Genetics (clinical)
Cell Proliferation
Gene knockdown
fungi
lcsh:RM1-950
Alternative splicing
RNA
Biomarker apoptosis
LncRNA
Long non-coding RNA
Up-Regulation
Cell biology
Gene Expression Regulation, Neoplastic
lcsh:Therapeutics. Pharmacology
030104 developmental biology
Bile Duct Neoplasms
030220 oncology & carcinogenesis
Molecular Medicine
Female
RNA, Long Noncoding
Carcinogenesis
Neoplasm Transplantation
Research Article
Subjects
Details
- ISSN :
- 15283658 and 10761551
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine
- Accession number :
- edsair.doi.dedup.....c762287da3988e194bf190585805c433