Back to Search
Start Over
Transplantation of feeder-free human induced pluripotent stem cell-derived cortical neuron progenitors in adult male Wistar rats with focal brain ischemia
- Source :
- Journal of Neuroscience Research. 96:863-874
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- The use of human induced pluripotent stem cells (hiPSCs) eliminates the ethical issues associated with fetal or embryonic materials, thus allowing progress in cell therapy research for ischemic stroke. Strict regulation of cell therapy development requires the xeno-free condition to eliminate clinical complications. Maintenance of hiPSCs with feeder-free condition presents a higher degree of spontaneous differentiation in comparison with conventional cultures. Therefore, feeder-free derivation might be not ideal for developing transplantable hiPSC derivatives. We developed the feeder-free condition for differentiation of cortical neurons from hiPSCs. Then, we evaluated the cells' characteristics upon transplantation into the sham and focal brain ischemia on adult male Wistar rats. Grafts in lesioned brains demonstrated polarized reactivity toward the ischemic border, indicated by directional preferences in axonal outgrowth and cellular migration, with no influence on graft survival. Following the transplantation, forelimb asymmetry was better restored compared with controls. Herein, we provide evidence to support the use of the xeno-free condition for the development of cell therapy for ischemic stroke.
- Subjects :
- Male
0301 basic medicine
Induced Pluripotent Stem Cells
Cell Culture Techniques
Brain Ischemia
Cell Line
Cell therapy
Brain ischemia
Random Allocation
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Neural Stem Cells
Animals
Humans
Medicine
Rats, Wistar
Progenitor cell
Induced pluripotent stem cell
Cell Proliferation
Neurons
business.industry
Cell Differentiation
Cell migration
medicine.disease
Embryonic stem cell
Neural stem cell
Rats
Transplantation
030104 developmental biology
business
Neuroscience
030217 neurology & neurosurgery
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 03604012
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi.dedup.....c758ef9112797616ecee38eced660605