Back to Search
Start Over
Comparative Evaluation of Different Chitosan Species and Derivatives as Candidate Biomaterials for Oxygen-Loaded Nanodroplet Formulations to Treat Chronic Wounds
- Source :
- Marine Drugs, Volume 19, Issue 2, Marine Drugs, Vol 19, Iss 112, p 112 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Persistent hypoxia is a main clinical feature of chronic wounds. Intriguingly, oxygen-loaded nanodroplets (OLNDs), filled with oxygen-solving 2H,3H-decafluoropentane and shelled with polysaccharides, have been proposed as a promising tool to counteract hypoxia by releasing a clinically relevant oxygen amount in a time-sustained manner. Here, four different types of chitosan (low or medium weight (LW or MW), glycol-(G-), and methylglycol-(MG-) chitosan) were compared as candidate biopolymers for shell manufacturing. The aim of the work was to design OLND formulations with optimized physico-chemical characteristics, efficacy in oxygen release, and biocompatibility. All OLND formulations displayed spherical morphology, cationic surfaces, ≤500 nm diameters (with LW chitosan-shelled OLNDs being the smallest), high stability, good oxygen encapsulation efficiency, and prolonged oxygen release kinetics. Upon cellular internalization, LW, MW, and G-chitosan-shelled nanodroplets did not significantly affect the viability, health, or metabolic activity of human keratinocytes (HaCaT cell line). On the contrary, MG-chitosan-shelled nanodroplets showed very poor biocompatibility. Combining the physico-chemical and the biological results obtained, LW chitosan emerges as the best candidate biopolymer for future OLND application as a skin device to treat chronic wounds.
- Subjects :
- Chronic wound
Keratinocytes
Biocompatibility
Cell Survival
Kinetics
Pharmaceutical Science
chemistry.chemical_element
Biocompatible Materials
02 engineering and technology
engineering.material
Polysaccharide
Oxygen
Article
Chitosan
03 medical and health sciences
chemistry.chemical_compound
chitosan
chitosan-derivatives
chronic wound
human keratinocytes
nanodroplets
oxygen
Cell Hypoxia
HaCaT Cells
Humans
Molecular Weight
Nanoparticles
Particle Size
Wounds and Injuries
Drug Discovery
medicine
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
lcsh:QH301-705.5
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
021001 nanoscience & nanotechnology
HaCaT
chemistry
lcsh:Biology (General)
engineering
Biophysics
Biopolymer
medicine.symptom
0210 nano-technology
Subjects
Details
- Language :
- English
- ISSN :
- 16603397
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....c7548f21387f8568888f77198ba7f99e
- Full Text :
- https://doi.org/10.3390/md19020112