Back to Search
Start Over
Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe
- Source :
- Chemical Communications. 54:1849-1852
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.
- Subjects :
- Boron Compounds
0301 basic medicine
Time Factors
Structured illumination microscopy
010402 general chemistry
Tracking (particle physics)
01 natural sciences
Catalysis
Mice
03 medical and health sciences
symbols.namesake
chemistry.chemical_compound
Imaging, Three-Dimensional
Organelle
Materials Chemistry
Animals
Fluorescent Dyes
Organelles
Microscopy
Super-resolution microscopy
Chemistry
Macrophages
Metals and Alloys
General Chemistry
Golgi apparatus
Superresolution
Fluorescence
Hypochlorous Acid
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
RAW 264.7 Cells
030104 developmental biology
Ceramics and Composites
symbols
Biophysics
BODIPY
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....c735b8f5e77a35919af9d6718c37216a