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Differential change in root protein patterns of two wheat varieties under high and low nitrogen nutrition levels
- Source :
- Plant Science, Plant Science, Elsevier, 2005, 168, pp.81-87
- Publication Year :
- 2005
- Publisher :
- HAL CCSD, 2005.
-
Abstract
- Nitrogen deficiency tolerance is necessary to breed for wheat varieties adapted to low input management systems. The aim of this study was to identify root proteins differentially expressed in response to a nitrogen stress. Two wheat varieties were experimented at low (0.5 mM nitrate) and at high (3.0 mM nitrate) nitrogen levels. After 8 weeks, root samples were taken to assess enzyme activities and to perform two dimensional gel electrophoresis. A total of 860 root protein spots were qualitatively or quantitatively analysed. A significant variety effect existed for 74 spots, while a significant nitrate supply-dependent variation was observed for 56 spots. Moreover 20 spots showed a significant variety × N-supply effect. Correlations were made between physiological parameters (nitrogen content and enzyme activities) and the intensity of variable spots. Positive correlations were observed for nitrate reductase and glutamate dehydrogenase activities and the intensity of a number of root proteins. This approach may be a way to identify new structural or regulatory proteins involved in the response to the level of nitrogen fertilisation in different wheat varieties.
- Subjects :
- 0106 biological sciences
BAS NIVEAU D'INTRANT
chemistry.chemical_element
Plant Science
Biology
Nitrate reductase
01 natural sciences
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
chemistry.chemical_compound
Nitrate
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
Genetics
Poaceae
AGRONOMIE
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
2. Zero hunger
0303 health sciences
Two-dimensional gel electrophoresis
Spots
Nitrogen deficiency
Glutamate dehydrogenase
General Medicine
AMELIORATION DES PLANTES
Nitrogen
Horticulture
chemistry
Agronomy
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 01689452
- Database :
- OpenAIRE
- Journal :
- Plant Science, Plant Science, Elsevier, 2005, 168, pp.81-87
- Accession number :
- edsair.doi.dedup.....c73421bee45b58e69eb1add38d8784bb