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Activity of cilofungin (LY 121019), a new lipopeptide antibiotic, on the cell wall and cytoplasmic membrane ofCandida albicans. Structural modifications in scanning and transmission electron microscopy
- Source :
- Medical Mycology. 28:425-436
- Publication Year :
- 1990
- Publisher :
- Oxford University Press (OUP), 1990.
-
Abstract
- Cilofungin, a new biosemisynthetic analog of echinocandin B, inhibits the synthesis of beta-(1,3)-glucan resulting in severe modifications of the cell wall and cytoplasmic membrane of sensitive organisms. The morphological modifications to budding yeast cells, pseudomycelium, mycelium and germ tubes of Candida albicans were studied by scanning and transmission electron microscopy after 3 and 16 h exposure to cilofungin. Changes in yeast cell morphology were apparent after 3 h in 0.1 microgram ml-1 cilofungin but were more marked in 1 and 10 micrograms ml-1 cilofungin. Most of the yeasts failed to separate and formed aggregates. Cracks and discontinuities were present in the cell wall and the cell membrane became undulated and fractured. Inclusions into the periplasmalemma space were observed, along with a release of cellular components. An important inhibition of germ tube formation was noted and the structure of true mycelium and pseudomycelium was severely modified. The budding area of yeast cells was particularly susceptible to damage by cilofungin.
- Subjects :
- Cytoplasm
Antifungal Agents
Germ tube
Cell morphology
Peptides, Cyclic
Cell wall
Cell membrane
Echinocandins
chemistry.chemical_compound
Cell Wall
Echinocandin B
Candida albicans
medicine
biology
Cell Membrane
Cilofungin
General Medicine
biology.organism_classification
Yeast
Microscopy, Electron
Infectious Diseases
medicine.anatomical_structure
chemistry
Biochemistry
Microscopy, Electron, Scanning
Biophysics
Peptides
Subjects
Details
- ISSN :
- 14602709 and 13693786
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Medical Mycology
- Accession number :
- edsair.doi.dedup.....c72b2db9cc53f191eb52303b50d463ad