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Pin1 Is Involved in HDAC6-mediated Cancer Cell Motility
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2018
- Publisher :
- Ivyspring International Publisher, 2018.
-
Abstract
- Histone deacetylase 6 (HDAC6), a member of the HDAC enzymes, has been reported to play substantial roles in many cellular processes. Evidence shows that deregulation of HDAC6 may be involved in the progression of some cancers, neurodegenerative diseases, and inflammatory disorders. However, little is known regarding the effect of post-translational modification of HDAC6 on cellular localization and biological functions. In the present study, we identified four phosphorylation sites on HDAC6 under normal conditions by mass spectrometry analysis. Two phosphorylation sites, pSer22 and pSer412, are recognized as Pin1 (peptidyl-prolyl cis/trans isomerase NIMA-interacting 1) substrates. Pin1 can interact with HDAC6 and be involved in HDAC6-mediated cell motility. Pin1 depletion abrogates HDAC6-induced cell migration and invasion in H1299 lung cancer cells. The findings of this study suggest that Pin1 might regulate HDAC6-mediated cell motility through alteration of protein conformation and function. Our results indicate the complexity of activity regulation between HDAC6 and Pin1, expanding knowledge regarding the multifunctional roles of Pin1 in tumorigenesis and cancer progression.
- Subjects :
- 0301 basic medicine
Motility
Biology
migration
Histone Deacetylase 6
medicine.disease_cause
Mass Spectrometry
03 medical and health sciences
Cell Movement
Cell Line, Tumor
medicine
Humans
Phosphorylation
Cellular localization
Cancer
Cell migration
General Medicine
HDAC6
invasion
medicine.disease
Pin1
Cell biology
NIMA-Interacting Peptidylprolyl Isomerase
030104 developmental biology
Cancer cell
PIN1
Carcinogenesis
Research Paper
Subjects
Details
- ISSN :
- 14491907
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....c7228e0c3d323caebd5ce464bac66968