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Glucokinase neurons of the paraventricular nucleus of the thalamus sense glucose and decrease food consumption
- Source :
- iScience, iScience, Vol 24, Iss 10, Pp 103122-(2021)
-
Abstract
- Summary The paraventricular nucleus of the thalamus (PVT) controls goal-oriented behavior through its connections to the nucleus accumbens (NAc). We previously characterized Glut2aPVT neurons that are activated by hypoglycemia, and which increase sucrose seeking behavior through their glutamatergic projections to the NAc. Here, we identified glucokinase (Gck)-expressing neurons of the PVT (GckaPVT) and generated a mouse line expressing the Cre recombinase from the glucokinase locus (GckCre/+ mice). Ex vivo calcium imaging and whole-cell patch clamp recordings revealed that GckaPVT neurons that project to the NAc were mostly activated by hyperglycemia. Their chemogenetic inhibition or optogenetic stimulation, respectively, enhanced food intake or decreased sucrose-seeking behavior. Collectively, our results describe a neuronal population of Gck-expressing neurons in the PVT, which has opposite glucose sensing properties and control over feeding behavior than the previously characterized Glut2aPVT neurons. This study allows a better understanding of the complex regulation of feeding behavior by the PVT.<br />Graphical abstract<br />Highlights • Glucokinase (Gck) is expressed in neurons of the paraventricular thalamus (PVT) • Most Gck-expressing PVT neurons are glucose-excited neurons • Chemogenetic inhibition of the GckPVT neurons increases food intake • Optogenetic activation of accumbens-projecting GckPVT neurons decreases sucrose seeking<br />Behavioral neuroscience; Cellular neuroscience; Neuroscience
- Subjects :
- medicine.medical_specialty
Science
Thalamus
Nucleus accumbens
Optogenetics
Article
03 medical and health sciences
0302 clinical medicine
Calcium imaging
Cellular neuroscience
Internal medicine
medicine
Patch clamp
030304 developmental biology
0303 health sciences
Multidisciplinary
Chemistry
Glucokinase
Behavioral neuroscience
medicine.anatomical_structure
Endocrinology
nervous system
Nucleus
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 25890042
- Volume :
- 24
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- iScience
- Accession number :
- edsair.doi.dedup.....c718f2cc43b8f99ea85a417811f0cb9d
- Full Text :
- https://doi.org/10.1016/j.isci.2021.103122