Back to Search
Start Over
Highly efficient dual-channel cytometric-detection of micron-sized particles in microfluidic device
- Source :
- Sensors and Actuators B: Chemical. 151:402-409
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- To demonstrate the ability to efficiently count and identify suspended micron-sized particles by simultaneously detecting their fluorescence emission and light scattering in microfabricated channel, a compact configuration that used a polydimethylsiloxane (PDMS) microfabricated channel as interrogation component, hydrodynamic focusing for particle control, and a simple free-space optical setup for signal detection, was accordingly developed. Subsequently, a quantitative count of 1.013 μm diameter fluorescently labeled beads in suspension was implemented in a microfluidic device employing both fluorescence emission and light scattering at average particle throughput ranging from 83 to 416 particles/s. As a result, the detection efficiencies above 88% for both signals and correlation percentages above 97% between them were routinely achieved. In addition, it was shown that effective differentiation of 1.013 μm fluorescently labeled beads from various unlabeled beads in mixed populations of high mixing ratios had been successfully realized in this microfluidic-device-based instrumentation. Finally, the demonstrated system was used to detect fluorescein isothiocyanate (FITC) labeled nonpathogenic bacteria of Escherichia coli (E. coli) DH5α. The results showed the detection efficiencies above 89.7% for fluorescence emission and 94.5% for light scattering signals, and a correlation of 94.9% between the two signals at an average throughput of 350 cells/s have been obtained. As a comparison, the detection accuracies of the dual-channel cytometric detection of the FITC-labeled E. coli DH5α cells in the microfluidic device are approximately 84.3% and 88.8% for fluorescence emission and light scattering respectively when compared against a manual cell count using a haemocytometer as a standard. © 2010 Elsevier B.V. All rights reserved.
- Subjects :
- Microfabricated channels
Scattering signals
Microfluidics
Silicones
Analytical chemistry
Escherichia coli (E. coli)
Hydrodynamic focusing
Light scattering
Scattering
chemistry.chemical_compound
Materials Chemistry
Instrumentation
Fluorescein isothiocyanate
Metals and Alloys
Non-pathogenic bacterium
Cell count
Condensed Matter Physics
Fluorescence
Polydimethylsiloxane PDMS
Dual channel detections
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
High-throughput measurements
Microfabrication
Micron-sized particles
Materials science
Average throughput
Fluorescence emission
Free-space optical
Cytometric analysis
Detection efficiency
Fluidic devices
Hemocytometer
Escherichia coli
Electrical and Electronic Engineering
Chromatography
Dual channel
Polydimethylsiloxane
Suspensions (fluids)
E. coli
Mixing ratios
Throughput
Refraction
chemistry
Particle
Micro-fluidic devices
Particle control
Detection accuracy
Signal detection
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 151
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi.dedup.....c717ef2c4257fa0c1dd9c231f8879e79
- Full Text :
- https://doi.org/10.1016/j.snb.2010.09.055