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Cyanobacterial NOS expression improves nitrogen use efficiency, nitrogen-deficiency tolerance and yield in Arabidopsis
- Source :
- Plant Sci. 307:110860 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier Ireland Ltd, 2021.
-
Abstract
- Developing strategies to improve nitrogen (N) use efficiency (NUE) in plants is a challenge to reduce environmental problems linked to over-fertilization. The nitric oxide synthase (NOS) enzyme from the cyanobacteria Synechococcus PCC 7335 (SyNOS) has been recently identified and characterized. SyNOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO), and then 70% of the produced NO is rapidly oxidized to nitrate by an unusual globin domain in its 5'-terminus. In this study, we assessed whether SyNOS expression in plants affects N metabolism improving NUE and yield. Our results showed that transgenic Arabidopsis plants had higher primary shoot length and shoot branching when grown in N-deficient conditions and higher seed production in N-sufficient and -deficient conditions. Moreover, transgenic plants showed significantly increased NUE in both N conditions. No differences were observed in N uptake for SyNOS lines. However, SyNOS lines presented an increase in N assimilation/remobilization under low N conditions. In addition, SyNOS lines had greater N-deficiency tolerance compared to wt plants. Our results support that SyNOS expression generates a positive effect on N metabolism and seed production in Arabidopsis, and it might be envisaged as a strategy to improve productivity in crops under adverse N environments.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cyanobacteria
Arginine
Nitrogen
Arabidopsis
Plant Science
Genetically modified crops
Nitric Oxide
01 natural sciences
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Nitrate
Nitric Oxide Synthase
Nitrogen Deficiency Tolerance
Nitrogen Use Efficiency
Seed Yield
Synechococcus Pcc 7335
Genetics
Citrulline
biology
Nitrogen deficiency
fungi
food and beverages
General Medicine
Plants, Genetically Modified
biology.organism_classification
Horticulture
030104 developmental biology
chemistry
Shoot
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Plant Sci. 307:110860 (2021)
- Accession number :
- edsair.doi.dedup.....c71217a5ea9d511fecd31ed753dc8e6e