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Aquaporin-4-dependent glymphatic solute transport in the rodent brain
- Source :
- eLife, eLife, Vol 7 (2018), Mestre, H, Hablitz, L M, Xavier, A L R, Feng, W, Zou, W, Pu, T, Monai, H, Murlidharan, G, Rivera, R M C, Simon, M J, Pike, M M, Pla, V, Du, T, Kress, B T, Wang, X, Plog, B A, Thrane, A S, Lundgaard, I, Abe, Y, Yasui, M, Thomas, J H, Xiao, M, Hirase, H, Asokan, A, Iliff, J J & Nedergaard, M 2018, ' Aquaporin-4-dependent glymphatic solute transport in the rodent brain ', eLife, vol. 7, :e40070 . https://doi.org/10.7554/eLife.40070
- Publication Year :
- 2018
-
Abstract
- The glymphatic system is a brain-wide clearance pathway; its impairment contributes to the accumulation of amyloid-β. Influx of cerebrospinal fluid (CSF) depends upon the expression and perivascular localization of the astroglial water channel aquaporin-4 (AQP4). Prompted by a recent failure to find an effect of Aqp4 knock-out (KO) on CSF and interstitial fluid (ISF) tracer transport, five groups re-examined the importance of AQP4 in glymphatic transport. We concur that CSF influx is higher in wild-type mice than in four different Aqp4 KO lines and in one line that lacks perivascular AQP4 (Snta1 KO). Meta-analysis of all studies demonstrated a significant decrease in tracer transport in KO mice and rats compared to controls. Meta-regression indicated that anesthesia, age, and tracer delivery explain the opposing results. We also report that intrastriatal injections suppress glymphatic function. This validates the role of AQP4 and shows that glymphatic studies must avoid the use of invasive procedures.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Rodent
Mouse
QH301-705.5
Science
aquaporin-4
General Biochemistry, Genetics and Molecular Biology
cerebrospinal fluid
03 medical and health sciences
0302 clinical medicine
Cerebrospinal fluid
Interstitial fluid
biology.animal
Internal medicine
medicine
Animals
Biology (General)
replication study
Aquaporin 4
Mice, Knockout
General Immunology and Microbiology
biology
General Neuroscience
glymphatic
Brain
Biological Transport
Extracellular Fluid
General Medicine
Anatomy
Rats
meta-analysis
030104 developmental biology
Endocrinology
Water channel
Astrocytes
solute transport
Medicine
Glymphatic system
sense organs
Glymphatic System
030217 neurology & neurosurgery
Research Article
Neuroscience
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....c6ffadb4a579883c6532d272a692b56e