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In vivo vascularization and islet function in a microwell device for pancreatic islet transplantation
- Source :
- Biomedical Materials (Bristol), 16(3):035036. Institute of Physics (IOP), Biomedical Materials (Bristol, England), 16(3):035036. IOP PUBLISHING LTD
- Publication Year :
- 2021
-
Abstract
- Islet encapsulation in membrane-based devices could allow for transplantation of donor islet tissue in the absence of immunosuppression. To achieve long-term survival of islets, the device should allow rapid exchange of essential nutrients and be vascularized to guarantee continued support of islet function. Recently, we have proposed a membrane-based macroencapsulation device consisting of a microwell membrane for islet separation covered by a micropatterned membrane lid. The device can prevent islet aggregation and support functional islet survival in vitro. Here, based on previous modeling studies, we develop an improved device with smaller microwell dimensions, decreased spacing between the microwells and reduced membrane thickness and investigate its performance in vitro and in vivo. This improved device allows for encapsulating higher islet numbers without islet aggregation and by applying an in vivo imaging system we demonstrate very good perfusion of the device when implanted intraperitoneally in mice. Besides, when it is implanted subcutaneously in mice, islet viability is maintained and a vascular network in close proximity to the device is developed. All these important findings demonstrate the potential of this device for islet transplantation.
- Subjects :
- microwell device
endocrine system
endocrine system diseases
macroencapsulation
0206 medical engineering
Biomedical Engineering
UT-Hybrid-D
Bioengineering
Membrane thickness
02 engineering and technology
Biomaterials
vascularization
In vivo
geography
geography.geographical_feature_category
Chemistry
islet transplantation
021001 nanoscience & nanotechnology
Islet
020601 biomedical engineering
In vitro
Cell biology
Transplantation
Vascular network
Pancreatic islet transplantation
0210 nano-technology
Function (biology)
Subjects
Details
- Language :
- English
- ISSN :
- 17486041
- Volume :
- 16
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedical Materials (Bristol, England)
- Accession number :
- edsair.doi.dedup.....c6effea57e53fed412a75e3d4b9c0c6e