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Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-d-aspartate Receptor Trafficking during Synaptic Plasticity
- Source :
- Journal of Biological Chemistry. 290:25395-25410
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- N-Methyl-d-aspartate receptor (NMDAR) synaptic incorporation changes the number of NMDARs at synapses and is thus critical to various NMDAR-dependent brain functions. To date, the molecules involved in NMDAR trafficking and the underlying mechanisms are poorly understood. Here, we report that myosin IIb is an essential molecule in NMDAR synaptic incorporation during PKC- or θ burst stimulation-induced synaptic plasticity. Moreover, we demonstrate that myosin light chain kinase (MLCK)-dependent actin reorganization contributes to NMDAR trafficking. The findings from additional mutual occlusion experiments demonstrate that PKC and MLCK share a common signaling pathway in NMDAR-mediated synaptic regulation. Because myosin IIb is the primary substrate of MLCK and can regulate actin dynamics during synaptic plasticity, we propose that the MLCK- and myosin IIb-dependent regulation of actin dynamics is required for NMDAR trafficking during synaptic plasticity. This study provides important insights into a mechanical framework for understanding NMDAR trafficking associated with synaptic plasticity.
- Subjects :
- Myosin light-chain kinase
endocrine system diseases
macromolecular substances
In Vitro Techniques
Biology
Hippocampus
Receptors, N-Methyl-D-Aspartate
Biochemistry
Rats, Sprague-Dawley
Mice
Neurobiology
mental disorders
Myosin
Neuroplasticity
Animals
Myosin-Light-Chain Kinase
Molecular Biology
Protein Kinase C
Protein kinase C
Actin
Mice, Knockout
Neuronal Plasticity
Nonmuscle Myosin Type IIB
musculoskeletal, neural, and ocular physiology
nutritional and metabolic diseases
Cell Biology
Actins
Rats
Cell biology
Enzyme Activation
Protein Transport
nervous system
Synaptic plasticity
NMDA receptor
Signal transduction
hormones, hormone substitutes, and hormone antagonists
psychological phenomena and processes
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 290
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....c6ea24d020848373bef62e46aa807d96