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Strategies to model AL amyloidosis in mice
- Source :
- Amyloid: The Journal of Protein Folding Disorders, Amyloid: The Journal of Protein Folding Disorders, Taylor & Francis, 2011, 18 (Suppl 1), pp.45-47. ⟨10.3109/13506129.2011.574354016⟩, ResearcherID
- Publication Year :
- 2011
- Publisher :
- Informa UK Limited, 2011.
-
Abstract
- International audience; Monoclonal immunoglobulin (Ig) deposition in AL amyloidosis is a severe complication of lymphoproliferative disorders. Research on this disease suffers from the lack of animal models, as they could allow for the testing of new innovative therapeutic strategies. We are trying to develop a transgenic animal model for this disease by overexpressing Ig light chain (LC) sequences cloned from AL amyloidosis patients. After several unsuccessful attempts using additional transgenesis due to low LC expression, we currently are following a strategy of targeted insertion of human LC sequences in the mouse endogenous kappa LC locus. We describe here the first data from this ‘knock-in’ model and propose future prospects to increase the rates of free LC and mimic the human disease. Absence of amyloid deposits in such models would raise the possibility of a resistance to AL amyloidosis in mice and question the feasibility of a reliable animal model for this disease.
- Subjects :
- MESH: DNA Primers
NLM
Transgene
Lymphoproliferative disorders
Disease
MESH: Base Sequence
Biology
Immunoglobulin light chain
Polymerase Chain Reaction
Mass Spectrometry
law.invention
Mice
03 medical and health sciences
0302 clinical medicine
law
Internal Medicine
medicine
AL amyloidosis
Animals
MESH: Amyloidosis
MESH: Animals
MESH: Mice
Severe complication
Polymerase chain reaction
DNA Primers
030304 developmental biology
MESH: Mass Spectrometry
0303 health sciences
Base Sequence
[CHIM.ORGA]Chemical Sciences/Organic chemistry
MESH: Polymerase Chain Reaction
Amyloidosis
medicine.disease
3. Good health
Transgenesis
Disease Models, Animal
030220 oncology & carcinogenesis
Immunology
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Disease Models, Animal
Subjects
Details
- ISSN :
- 17442818 and 13506129
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Amyloid
- Accession number :
- edsair.doi.dedup.....c6d995b88698991ea7ac8c0f0b057dff
- Full Text :
- https://doi.org/10.3109/13506129.2011.574354016