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Ultrasound-targeted microbubble destruction-mediated microRNA-21 transfection regulated PDCD4/NF-κB/TNF-α pathway to prevent coronary microembolization-induced cardiac dysfunction
- Source :
- Gene Therapy. 22:1000-1006
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- The programmed cell death 4/nuclear factor-κB/tumor necrosis factor α (PDCD4/NF-κB/TNF-α) signaling pathway has an important role in coronary microembolization (CME)-induced inflammation. microRNA-21 protects myocardium mainly via regulation of its target gene PDCD4. Therefore, in this study we investigated the effect of ultrasound-guided microbubble-mediated microRNA-21 transfection on cardiac function in CME pig model and determined the potential mechanisms involved. The pig CME model was established by microcatheter-mediated injection of microembolization beads into the left anterior descending artery. The CME with microRNA transfection group was injected with plasmid-microbubble mixture through the marginal ear vein 4 days before CME treatment, along with ultrasound to the myocardium. Cardiac function indices were examined by cardiac ultrasound; infarct area was measured by hematoxylin-eosin and hematoxylin-basic Fuchsin-picric acid staining of tissue pathological sections; green fluorescent protein-labeled gene expression levels were evaluated by fluorescent microscopy in frozen sections; myocardial PDCD4 and TNF-α mRNA levels were measured by fluorescent quantitative PCR and protein levels were measured by western blotting; and NF-κB activity was tested by electrophoretic mobility shift assay. Compared with the CME group, the CME with ultrasound-mediated microRNA transfection group demonstrated improved CME-induced cardiac dysfunction (P
- Subjects :
- Cardiac function curve
Programmed cell death
Swine
education
Coronary Disease
Cell Communication
Biology
Transfection
Gene expression
microRNA
Genetics
Animals
Myocyte
Myocytes, Cardiac
Molecular Biology
Microbubbles
Tumor Necrosis Factor-alpha
Myocardium
NF-kappa B
RNA-Binding Proteins
Cell Differentiation
Coronary Vessels
Molecular biology
MicroRNAs
Real-time polymerase chain reaction
Swine, Miniature
Molecular Medicine
Tumor necrosis factor alpha
Apoptosis Regulatory Proteins
Cardiomyopathies
Signal Transduction
Subjects
Details
- ISSN :
- 14765462 and 09697128
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Gene Therapy
- Accession number :
- edsair.doi.dedup.....c6d4e321725390476e41955b0bf61239