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'Multiple-Particle-Tracking to investigate viscoelastic properties in living cells'
- Publication Year :
- 2010
-
Abstract
- Cell mechanical properties play an important role in determining many cellular activities. Passive microrheology techniques, such as Multiple-Particle-Tracking (MPT) give an insight into the structural rearrangements and viscoelastic response of a wide range of materials, in particular soft materials and complex fluids like cell cytoplasm in living cells. The technique finds an important field of application in large cells such as oocytes where, during their growth, several organelles and molecules are displaced in specific territories of the cell instrumental for later embryonic development. To measure cell mechanics, cells are usually deformed by many techniques that are slow and often invasive. To overcome these limits, the MPT technique is applied. Probe particles are embedded in the viscoelastic sample and their properties are extracted from the thermal fluctuation spectra measured using digital video-microscopy. The Brownian motion of a probe particle immersed in a network is directly related to the network's mechanical properties. Particles exhibit larger motions when their local environments are less rigid or less viscous. The mean-square-displacement (MSD) of the particle's trajectory is used to quantify its amplitude of motions over different time scales.
- Subjects :
- Microrheology
Cytoplasm
Materials science
Multiple-Particle-Tracking
Cytological Techniques
Biophysics
xenopus laevi
Nanotechnology
living cell
Mitochondrial cloud
Models, Biological
General Biochemistry, Genetics and Molecular Biology
Viscoelasticity
Quantitative Biology::Cell Behavior
Xenopus laevis
Rheology
Chlorocebus aethiops
Animals
viscoelastic propertie
oocytes
Particle Size
Molecular Biology
Brownian motion
Complex fluid
Microscopy, Video
Viscosity
Elasticity
COS Cells
Particle size
Oligonucleotide Probes
Biological system
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c6c65b1d404542fc37f9e5037fae1261