成熟雌ニホンツキノワグマ（Ursus thibetanus japonicus）における血清レプチン濃度の周年変化

The assay of serum leptin concentrations in the Japanese black bear (Ursus thibetanus japonicus) was tested by canine-leptin-specific sandwich enzymelinked immunosorbent assay (ELISA). A dose-response curve of the bear serum was linear and parallel to the canine leptin standard curve. In mated and unmated bears, the serum leptin concentration was stable at low levels from May to August or September and a gradual increase from September or October, followed by a remarkable increase in late November. We conclude that this method may be useful for measuring bear serum leptin concentrations and that serum leptin concentrations change annually with a peak in late November.
イヌレプチン特異的酵素抗体測定法により、ニホンツキノワグマ（以下クマと略）の血清レプチン濃度測定を試みた。交尾群および非交尾群の血清レプチン濃度は、5～8 または9 月に低レベルで推移し、9 または10 月から徐々に増加し11 月下旬に顕著に上昇した。本測定法はクマ血清レプチン濃度測定に有効性が示唆され、レプチン濃度は11 月下旬にピークを有する明瞭な周年変化を示すことが明らかとなった。