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Density gradient characterization of the high density lipoproteins in cholesterol-fed hyper- and hyporesponding patas monkeys (Erythrocebus patas)
- Source :
- Journal of Lipid Research, Vol 25, Iss 9, Pp 979-990 (1984)
- Publication Year :
- 1984
- Publisher :
- Elsevier BV, 1984.
-
Abstract
- Diet-induced changes in high density lipoprotein (HDL) density and size were studied in patas monkeys. When the animals were switched from a moderate fat-low cholesterol diet to a high fat-high cholesterol (HFHC) diet, the plasma apoA-I levels increased initially in all of the animals. The apoA-I levels remained elevated in monkeys able to maintain their plasma cholesterol concentrations near basal levels (hyporesponders), but began to decrease in monkeys who became severely hypercholesterolemic (hyperresponders), reaching levels as low as 65-70% of their basal value by 24 weeks. The larger, lipid-rich HDL (HDL2) was shown by density gradient ultracentrifugation and gradient-PAGE (polyacrylamide gel electrophoresis) to be the HDL fraction responsible for these changes in apoA-I, completely accounting for the increase in apoA-I in hyporesponders and the decrease in apoA-I in hyperresponders. The HDL3 levels remained unchanged in hyporesponders but increased markedly in hyperresponders, partially compensating for the decrease of HDL2 in those animals. Gradient-PAGE showed the HDL3 to be heterogeneous, containing at least two populations of particles of the same density but differing significantly in size. The smaller of these HDL3 were most prominent in the HFHC-fed hyperresponders. These data show that nonhuman primate HDL is both physically and metabolically heterogeneous, and indicate that a high fat-high cholesterol diet-induced hypercholesterolemia severely depresses the HDL2 levels.
- Subjects :
- medicine.medical_specialty
Density gradient
biology
Cholesterol
Erythrocebus patas
nutritional and metabolic diseases
QD415-436
Cell Biology
Apolipoproteins A
biology.organism_classification
Biochemistry
chemistry.chemical_compound
Basal (phylogenetics)
Endocrinology
High-density lipoprotein
chemistry
Patas monkey
Internal medicine
medicine
lipids (amino acids, peptides, and proteins)
Density gradient ultracentrifugation
Subjects
Details
- ISSN :
- 00222275
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....c6b6e45815218a1d3434b5174408612e