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The duck hepatitis virus 5'-UTR possesses HCV-like IRES activity that is independent of eIF4F complex and is modulated by downstream coding sequences
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Virology Journal, Vol 8, Iss 1, p 147 (2011), Virology Journal
- Publication Year :
- 2011
- Publisher :
- BioMed Central, 2011.
-
Abstract
- Duck hepatitis virus (DHV-1) is a worldwide distributed picornavirus that causes acute and fatal disease in young ducklings. Recently, the complete genome of DHV-1 has been determined and comparative sequence analysis has shown that possesses the typical picornavirus organization but exhibits several unique features. For the first time, we provide evidence that the 626-nucleotide-long 5'-UTR of the DHV-1 genome contains an internal ribosome entry site (IRES) element that functions efficiently both in vitro and in mammalian cells. The prediction of the secondary structure of the DHV-1 IRES shows significant similarity to the hepatitis C virus (HCV) IRES. Moreover, similarly to HCV IRES, DHV-1 IRES can direct translation initiation in the absence of a functional eIF4F complex. We also demonstrate that the activity of the DHV-1 IRES is modulated by a viral coding sequence located downstream of the DHV-1 5'-UTR, which enhances DHV-1 IRES activity both in vitro and in vivo. Furthermore, mutational analysis of the predicted pseudo-knot structures at the 3'-end of the putative DHV-1 IRES supported the presence of conserved domains II and III and, as it has been previously described for other picornaviruses, these structures are essential for keeping the normal internal initiation of translation of DHV-1.<br />This study was funded by grants from the Chinese Natural Sciences Foundation (30870114), National Key Laboratory of Veterinary Biotechnology (SKLVBF201004), the Special Fund for Agro-scientific Research in the Public Interest (No.201003012), the National advanced Technology Research and Development Program of China (863 Program) (No. 2011AA10A200).
- Subjects :
- Gene Expression Regulation, Viral
Untranslated region
Five prime untranslated region
Picornavirus
Molecular Sequence Data
Genome, Viral
Hepacivirus
Duck hepatitis virus
Hepatitis Virus, Duck
Cell Line
lcsh:Infectious and parasitic diseases
Eukaryotic translation
Cricetinae
Virology
Animals
Coding region
lcsh:RC109-216
Conserved Sequence
Genetics
Picornaviridae Infections
Base Sequence
biology
Research
fungi
Translation (biology)
biology.organism_classification
Internal ribosome entry site
Infectious Diseases
Eukaryotic Initiation Factor-4F
Hepatitis, Viral, Animal
Protein Biosynthesis
Nucleic Acid Conformation
RNA, Viral
5' Untranslated Regions
Chickens
Protein Binding
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Virology Journal, Vol 8, Iss 1, p 147 (2011), Virology Journal
- Accession number :
- edsair.doi.dedup.....c6b407c5dbda422cfad3fed91c6e2ec3