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PINCH1 regulates cell-matrix and cell-cell adhesions, cell polarity and cell survival during the peri-implantation stage
- Source :
- Journal of cell science. 118(Pt 13)
- Publication Year :
- 2005
-
Abstract
- PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking β1 integrin or Ilk, loss of PINCH1 arrested development at the peri-implantation stage. In contrast to β1 integrin or Ilk mutants, however, disruption of the PINCH1 gene produced implantation chambers with visible cell clumps even at embryonic day 9.5. In order to define the phenotype leading to the peri-implantation lethality we made PINCH1-null EBs and found similar but also additional defects not observed in β1 integrin or Ilk mutant EBs. The similarities included abnormal epiblast polarity, impaired cavitation and detachment of endoderm and epiblast from basement membranes. Additional defects, which were not observed in β1 integrin- or ILK-deficient mice or EBs, included abnormal cell-cell adhesion of endoderm and epiblast as well as the presence of apoptotic cells in the endodermal cell layer. Although ILK and PINCH1 were shown to be involved in the phosphorylation of serine-473 of PKB/Akt, immunostaining with specific antibodies revealed no apparent alteration of PKB/Akt phosphorylation in PINCH1-deficient EBs. Altogether these data demonstrate an important role of PINCH1 for integrin function, actin organization, cell-cell adhesion and endodermal cell survival during the implanting of mouse embryos.
- Subjects :
- Cell Survival
Integrin
Molecular Sequence Data
Apoptosis
Embryoid body
Tight Junctions
Extracellular matrix
Mice
Cell polarity
medicine
Cell Adhesion
Animals
Embryo Implantation
Protein kinase B
Adaptor Proteins, Signal Transducing
biology
Cell Polarity
Membrane Proteins
Cell Biology
LIM Domain Proteins
Embryo, Mammalian
Embryonic stem cell
Actins
Cell biology
Extracellular Matrix
DNA-Binding Proteins
medicine.anatomical_structure
Epiblast
embryonic structures
biology.protein
Endoderm
Subjects
Details
- ISSN :
- 00219533
- Volume :
- 118
- Issue :
- Pt 13
- Database :
- OpenAIRE
- Journal :
- Journal of cell science
- Accession number :
- edsair.doi.dedup.....c6b136b6a0fb6dd18de9a6e354355274