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Temporal changes of light-induced proteins in the SCN following treatment with the serotonin mixed agonist/antagonist BMY7378
- Source :
- Experimental Brain Research. 233:2723-2731
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- The 5-HT1A mixed agonist/antagonist BMY7378 has been shown to greatly potentiate photic phase advances in hamsters. The underlying mechanism and intracellular changes in the suprachiasmatic nucleus (SCN) by which this potentiation is accomplished have yet to be fully determined. Here, we examine the effect of BMY7378 on temporal activation patterns of a number of proteins and enzymes in the SCN following light exposure in the late subjective night. BMY7378 administration increased the amount of several photo-inducible proteins in the SCN at specific time points following light exposure in the late subjective night. Relative to animals given saline before a light pulse, the number of cells immunoreactive for cFos, JunB and PER1 was all significantly greater 360 min following the light pulse in BMY7378 pretreated animals, indicating an extended action of these light-induced proteins in the SCN following BMY7378 pretreatment. Aside from a modest, nonsignificant increase in P-ERK levels at 60 min, BMY7378 did not affect light-induced P-ERK levels. The levels of light-induced P-CREB were similarly unaffected by BMY7378. Also unaffected by BMY7378 treatment were cFos expression and JunB expression at 120 and 180 min following light exposure. These findings suggest that BMY7378 may potentiate photic phase shifts at least partly by prolonging the activity of some, but not all, light-induced proteins and biochemical pathways involved in coupling the light signal to the output of the circadian clock, particularly those which are active many hours after the light signal reaches the SCN.
- Subjects :
- Male
Agonist
Calbindins
medicine.medical_specialty
Time Factors
Light
Agonist-antagonist
JUNB
medicine.drug_class
Circadian clock
Piperazines
Serotonin Agents
Cricetinae
Internal medicine
medicine
Animals
Circadian rhythm
Suprachiasmatic nucleus
Chemistry
General Neuroscience
Antagonist
Period Circadian Proteins
Circadian Rhythm
Endocrinology
Gene Expression Regulation
Suprachiasmatic Nucleus
sense organs
Proto-Oncogene Proteins c-fos
Transcription Factors
PER1
Subjects
Details
- ISSN :
- 14321106 and 00144819
- Volume :
- 233
- Database :
- OpenAIRE
- Journal :
- Experimental Brain Research
- Accession number :
- edsair.doi.dedup.....c6accc1693ee2ff692c7412a5e2e6507