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Mechanism-Oriented Controllability of Intracellular Quantum Dots Formation: The Role of Glutathione Metabolic Pathway
- Source :
- ACS Nano. 7:2240-2248
- Publication Year :
- 2013
- Publisher :
- American Chemical Society (ACS), 2013.
-
Abstract
- Microbial cells have shown a great potential to biosynthesize inorganic nanoparticles within their orderly regulated intracellular environment. However, very little is known about the mechanism of nanoparticle biosynthesis. Therefore, it is difficult to control intracellular synthesis through the manipulation of biological processes. Here, we present a mechanism-oriented strategy for controlling the biosynthesis of fluorescent CdSe quantum dots (QDs) by means of metabolic engineering in yeast cells. Using genetic techniques, we demonstrated that the glutathione metabolic pathway controls the intracellular CdSe QD formation. Inspired from this mechanism, the controllability of CdSe QD yield was realized through engineering the glutathione metabolism in genetically modified yeast cells. The yeast cells were homogeneously transformed into more efficient cell-factories at the single-cell level, providing a specific way to direct the cellular metabolism toward CdSe QD formation. This work could provide the foundation for the future development of nanomaterial biosynthesis.
- Subjects :
- Saccharomyces cerevisiae Proteins
Glutamate-Cysteine Ligase
General Physics and Astronomy
Nanoparticle
Saccharomyces cerevisiae
Biology
Metabolic engineering
chemistry.chemical_compound
Biosynthesis
Quantum Dots
Cadmium Compounds
Nanotechnology
General Materials Science
Selenium Compounds
General Engineering
Glutathione
Yeast
Metabolic pathway
Metabolic Engineering
Microscopy, Fluorescence
Biochemistry
chemistry
Quantum dot
Biophysics
Metabolic Networks and Pathways
Intracellular
Subjects
Details
- ISSN :
- 1936086X and 19360851
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- ACS Nano
- Accession number :
- edsair.doi.dedup.....c6ab69a3d46740fd665b8cd8227c4a78
- Full Text :
- https://doi.org/10.1021/nn305346a