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Inability of memory T cells to induce graft-versus-host disease is a result of an abortive alloresponse
- Source :
- Blood. 109:3115-3123
- Publication Year :
- 2006
- Publisher :
- American Society of Hematology, 2006.
-
Abstract
- Several groups, including our own, have independently demonstrated that effector memory T cells from non–alloantigen-primed donors do not cause graft-versus-host disease (GVHD). In the current study, we further investigated whether this approach could be extended to all memory T cells, and we studied the underlying mechanisms. Neither total memory T cells nor purified central memory T cells were able to induce GVHD. Memory T cells were at least 3-log less potent than bulk T cells in mediating GVHD. As expected, memory T cells failed to elicit cytotoxicity and proliferated poorly against alloantigens in standard 5-day mixed-lymphocyte cultures. However, the proliferative responses of memory T cells were more comparable with those of bulk and naive T cells when the culture time was shortened. Moreover, the frequencies of IL-2–secreting cells measured by 42-hour enzyme-linked immunosorbent spot (ELISPOT) assay were similar among naive, memory, and bulk T cells. These data indicated that memory T cells are able to respond to alloantigens initially but fail to develop to full potential. The abortive immune response, which was mediated by non–alloantigen-specific memory T cells in response to alloantigens, may explain why memory T cells from unprimed and non–alloantigen-primed donors could not induce GVHD.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
Isoantigens
Immunology
Graft vs Host Disease
chemical and pharmacologic phenomena
In Vitro Techniques
Biology
Biochemistry
Mice
Interleukin 21
Immune system
Animals, Congenic
T-Lymphocyte Subsets
medicine
Animals
Cytotoxic T cell
Mice, Inbred BALB C
Mice, Inbred C3H
Transplantation
ELISPOT
CD28
Cell Biology
Hematology
T lymphocyte
medicine.disease
Mice, Inbred C57BL
surgical procedures, operative
Graft-versus-host disease
Hemocyanins
Female
Lymphocyte Culture Test, Mixed
Stem cell
Immunologic Memory
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....c6ab1a55d193c980ccd0ccf86137aa90