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Rapid Determination of Quinolone Resistance in Acinetobacter spp
- Source :
- Journal of Clinical Microbiology. 47:1436-1442
- Publication Year :
- 2009
- Publisher :
- American Society for Microbiology, 2009.
-
Abstract
- In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized multidrug-resistant (MDR) Acinetobacter sp. isolates, we show that PCR followed by electrospray ionization mass spectrometry (PCR/ESI-MS) and base composition analysis of PCR amplification products can quickly and accurately identify quinolone resistance mediated by mutations in the quinolone resistance-determining regions of gyrA and parC , two essential housekeeping genes. Single point mutations detected by PCR/ESI-MS in parC (found in 55/75 of the isolates) and in gyrA (found in 66/75 of the isolates) correlated with susceptibility testing and sequencing. By targeting resistance determinants that are encoded by genes with highly conserved DNA sequences (e.g., gyrA and parC ), we demonstrate that PCR/ESI-MS can provide critical information for resistance determinant identification and can inform therapeutic decision making in the treatment of Acinetobacter sp. infections.
- Subjects :
- DNA Topoisomerase IV
DNA, Bacterial
Microbiology (medical)
Spectrometry, Mass, Electrospray Ionization
medicine.drug_class
Molecular Sequence Data
Antibiotics
Mutation, Missense
Microbial Sensitivity Tests
Quinolones
Polymerase Chain Reaction
law.invention
Microbiology
Bacterial Proteins
law
Drug Resistance, Bacterial
Antimicrobial chemotherapy
medicine
Humans
Point Mutation
Polymerase chain reaction
Acinetobacter
Base Sequence
biology
Bacteriology
biology.organism_classification
Quinolone
Housekeeping gene
Multiple drug resistance
DNA Gyrase
Neisseriaceae
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....c68e10ffb3011cbc4d1120c89fb9a7df