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Prevalence and Abundance of Florfenicol and Linezolid Resistance Genes in Soils Adjacent to Swine Feedlots
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Florfenicol is extensively used in livestock to prevent or cure bacterial infections. However, it is not known whether the administration of florfenicol has resulted in the emergence and dissemination of florfenicol resistance genes (FRGs, including fexA, fexB, cfr, optrA, floR and pexA) in microbial populations in surrounding farm environments. Here we collected soil samples for the detection of FRGs and the residue of florfenicol from six swine farms with the record of florfenicol usage. Quantitative polymerase chain reaction and metagenomic sequencing revealed a significantly higher relative abundance of FRGs in the soils adjacent to the three swine farms where florfenicol was heavily used compared with the other sites. Meanwhile, the detectable levels of florfenicol were also identified in soils from two of these three farms using ultra-performance liquid chromatography tandem mass spectrometry. It appears that amount of florfenicol used on swine farms and the spreading of soils with swine waste could promote the prevalence and abundance of FRGs, including the linezolid resistance genes cfr and optrA, in adjacent soils and agricultural application of swine manure with florfenicol may have caused a residual level of florfenicol in the soils.
- Subjects :
- 0301 basic medicine
Florfenicol
Veterinary medicine
Swine
animal diseases
Microorganism
030106 microbiology
Biology
Article
03 medical and health sciences
chemistry.chemical_compound
Drug Resistance, Bacterial
Animals
Pig farming
Relative species abundance
Soil Microbiology
Feces
Thiamphenicol
Multidisciplinary
business.industry
Linezolid
Animal Feed
Manure
Biotechnology
chemistry
Genes, Bacterial
Livestock
business
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....c65a7c73ac538c5b09675d7b60a424c1