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Functional Interaction of the Active Zone Proteins Munc13-1 and RIM1 in Synaptic Vesicle Priming
- Source :
- Neuron. 30:183-196
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca2+-triggered fusion are taking place in a highly coordinated manner. We show that the active zone components Munc13-1, an essential vesicle priming protein, and RIM1, a Rab3 effector with a putative role in vesicle tethering, interact functionally. Disruption of this interaction causes a loss of fusion-competent synaptic vesicles, creating a phenocopy of Munc13-1-deficient neurons. RIM1 binding and vesicle priming are mediated by two distinct structural modules of Munc13-1. The Munc13-1/RIM1 interaction may create a functional link between synaptic vesicle tethering and priming, or it may regulate the priming reaction itself, thereby determining the number of fusion-competent vesicles.
- Subjects :
- Vesicle fusion
Neuroscience(all)
Molecular Sequence Data
Presynaptic Terminals
Nerve Tissue Proteins
Biology
Synaptic Transmission
Synaptic vesicle
Vesicle tethering
Fungal Proteins
Two-Hybrid System Techniques
Synaptic augmentation
Animals
Protein Isoforms
Cells, Cultured
Neurons
Neurotransmitter Agents
Binding Sites
Sequence Homology, Amino Acid
General Neuroscience
SNAP25
Zinc Fingers
Munc-18
Kiss-and-run fusion
rab3A GTP-Binding Protein
Protein Structure, Tertiary
Cell biology
DNA-Binding Proteins
Alternative Splicing
Synaptic Vesicles
Synaptic vesicle priming
Subjects
Details
- ISSN :
- 08966273
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....c62986695f77a32cf6249e9928f05ec2