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Macrophages Promote Circulating Tumor Cell-Mediated Local Recurrence Following Radiation Therapy in Immunosuppressed Patients
- Publication Year :
- 2018
-
Abstract
- Although radiotherapy (RT) decreases the incidence of locoregional recurrence in breast cancer, patients with triple-negative breast cancer (TNBC) have increased risk of local recurrence following breast-conserving therapy. The relationship between RT and local recurrence is unknown. Here, we tested the hypothesis that recurrence in some instances is due to the attraction of circulating tumor cells to irradiated tissues. To evaluate the effect of absolute lymphocyte count on local recurrence after RT in patients with TNBC, we analyzed radiation effects on tumor and immune cell recruitment to tissues in an orthotopic breast cancer model. Recurrent patients exhibited a prolonged low absolute lymphocyte count when compared with nonrecurrent patients following RT. Recruitment of tumor cells to irradiated normal tissues was enhanced in the absence of CD8+ T cells. Macrophages (CD11b+F480+) preceded tumor cell infiltration and were recruited to tissues following RT. Tumor cell recruitment was mitigated by inhibiting macrophage infiltration using maraviroc, an FDA-approved CCR5 receptor antagonist. Our work poses the intriguing possibility that excessive macrophage infiltration in the absence of lymphocytes promotes local recurrence after RT. This combination thus defines a high-risk group of patients with TNBC. Significance: This study establishes the importance of macrophages in driving tumor cell recruitment to sites of local radiation therapy and suggests that this mechanism contributes to local recurrence in women with TNBC that are also immunosuppressed. Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/15/4241/F1.large.jpg. Cancer Res; 78(15); 4241–52. ©2018 AACR.
- Subjects :
- 0301 basic medicine
Cancer Research
Receptors, CCR5
medicine.medical_treatment
Mice, Nude
Triple Negative Breast Neoplasms
CCR5 receptor antagonist
CD8-Positive T-Lymphocytes
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Immune system
Circulating tumor cell
Breast cancer
Cell Movement
Cell Line, Tumor
medicine
Animals
Humans
Mastectomy
Maraviroc
Retrospective Studies
Mice, Inbred BALB C
business.industry
Macrophages
medicine.disease
Neoplastic Cells, Circulating
Radiation therapy
030104 developmental biology
Oncology
chemistry
030220 oncology & carcinogenesis
Cancer research
Female
Neoplasm Recurrence, Local
business
Infiltration (medical)
CD8
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c627198c912f72a7ba956ee35bb23669