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Protective Effects of Licochalcone A Improve Airway Hyper-Responsiveness and Oxidative Stress in a Mouse Model of Asthma
- Source :
- Cells, Cells, Vol 8, Iss 6, p 617 (2019), Volume 8, Issue 6
- Publication Year :
- 2019
-
Abstract
- Licochalcone A was isolated from Glycyrrhiza uralensis and previously reported to have antitumor and anti-inflammatory effects. Licochalcone A has also been found to inhibit the levels of Th2-associated cytokines in the bronchoalveolar lavage fluid (BALF) of asthmatic mice. However, the molecular mechanism underlying airway inflammation and how licochalcone A regulates oxidative stress in asthmatic mice are elusive. In this study, we investigated whether licochalcone A could attenuate inflammatory and oxidative responses in tracheal epithelial cells, and whether it could ameliorate oxidative stress and airway inflammation in asthmatic mice. Inflammatory human tracheal epithelial (BEAS-2B) cells were treated with licochalcone A to evaluate oxidative responses and inflammatory cytokine levels. In addition, BALB/c mice were sensitized with ovalbumin (OVA) and injected intraperitoneally with licochalcone A (5 or 10 mg/kg). Licochalcone A significantly inhibited reactive oxygen species, eotaxin, and proinflammatory cytokines in BEAS-2B cells. Licochalcone A also decreased intercellular adhesion molecule 1 levels in inflammatory BEAS-2B cells, blocking monocyte cell adherence. We also found that licochalcone A significantly decreased oxidative responses, reduced malondialdehyde levels, and increased glutathione levels in the lungs of OVA-sensitized mice. Furthermore, licochalcone A decreased airway hyper-responsiveness, eosinophil infiltration, and Th2 cytokine production in the BALF. These findings suggest that licochalcone A alleviates oxidative stress, inflammation, and pathological changes by inhibiting Th2-associated cytokines in asthmatic mice and human tracheal epithelial cells. Thus, licochalcone A demonstrated therapeutic potential for improving asthma.
- Subjects :
- 0301 basic medicine
Licochalcone A
THP-1 Cells
medicine.medical_treatment
Pharmacology
medicine.disease_cause
chemistry.chemical_compound
0302 clinical medicine
Chalcones
Antibody Specificity
Malondialdehyde
oxidative stress
lcsh:QH301-705.5
Lung
Mice, Inbred BALB C
General Medicine
respiratory system
Glutathione
medicine.anatomical_structure
Cytokine
030220 oncology & carcinogenesis
Female
Collagen
Goblet Cells
medicine.symptom
Chemokines
Inflammation Mediators
Bronchoalveolar Lavage Fluid
airway hyper-responsiveness
Ovalbumin
Inflammation
Protective Agents
Article
Proinflammatory cytokine
03 medical and health sciences
medicine
Cell Adhesion
Respiratory Hypersensitivity
Animals
Humans
eosinophil
Hyperplasia
Monocyte
licochalcone A
Eosinophil
asthma
Eosinophils
Disease Models, Animal
030104 developmental biology
chemistry
lcsh:Biology (General)
Cyclooxygenase 2
Reactive Oxygen Species
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 20734409
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....c61b5468804fae9d95cf4f01daab1956