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Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma

Authors :
Jian‑Chun Wang
Hong‑Tao Jin
Feng Wang
Zi‑Qian Zhou
Han‑Qing Liu
Ya‑Wen An
Zhi‑Wen Li
Guang‑Yu Jiang
Source :
Oncology Reports
Publication Year :
2020
Publisher :
Spandidos Publications, 2020.

Abstract

The aim of the present study was to explore the antitumor effects of sinoporphyrin sodium (DVDMS)-mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms. The uptake of DVDMS by U-118 MG cells was detected by flow cytometry (FCM). A 630-nm semiconductor laser and 1-MHz ultrasound were used to perform PDT and SDT, respectively. Cell proliferation and apoptosis were evaluated using the Cell Counting Kit-8 assay, FCM and Hoechst 33258 staining, respectively. Western blot analysis was used to detect protein expression and phosphorylation levels. BALB/c nude mice were used to establish a xenograft model of U-118 MG cells. DVDMS was injected intravenously and PDT and SDT were performed 24 h later. An in vivo imaging system was used to evaluate the fluorescence of DVDMS, to measure tumor sizes, and to evaluate the therapeutic effects. The uptake of DVDMS by U-118 MG cells was optimal after 4 h. PDT and SDT following DVDMS injection significantly inhibited the proliferation and increased apoptosis of glioma cells in vitro (P

Details

ISSN :
17912431 and 1021335X
Database :
OpenAIRE
Journal :
Oncology Reports
Accession number :
edsair.doi.dedup.....c619781242c8433cedf1ee661e190963