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Coupling of spliceosome complexity to intron diversity
- Source :
- Curr Biol
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- SUMMARYWe determined that over 40 spliceosomal proteins are conserved between many fungal species and humans but were lost during the evolution of S. cerevisiae, an intron-poor yeast with unusually rigid splicing signals. We analyzed null mutations in a subset of these factors, most of which had not been investigated previously, in the intron-rich yeast Cryptococcus neoformans. We found they govern splicing efficiency of introns with divergent spacing between intron elements. Importantly, most of these factors also suppress usage of weak nearby cryptic/alternative splice sites. Among these, orthologs of GPATCH1 and the helicase DHX35 display correlated functional signatures and copurify with each other as well as components of catalytically active spliceosomes, identifying a conserved G-patch/helicase pair that promotes splicing fidelity. We propose that a significant fraction of spliceosomal proteins in humans and most eukaryotes are involved in limiting splicing errors, potentially through kinetic proofreading mechanisms, thereby enabling greater intron diversity.
- Subjects :
- Spliceosome
RNA Splicing
Saccharomyces cerevisiae
Computational biology
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Humans
RNA, Messenger
RNA Processing, Post-Transcriptional
030304 developmental biology
Genetics
Cryptococcus neoformans
0303 health sciences
biology
Intron
Helicase
RNA
biology.organism_classification
Introns
Yeast
Coupling (computer programming)
RNA splicing
Spliceosomes
biology.protein
Kinetic proofreading
General Agricultural and Biological Sciences
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 09609822
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Current Biology
- Accession number :
- edsair.doi.dedup.....c61563bbf824602886369630fb7f561c