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A molecular-based identification resource for the arthropods of Finland
- Source :
- Molecular Ecology Resources
- Publication Year :
- 2022
- Publisher :
- John Wiley & Sons, 2022.
-
Abstract
- Publisher Copyright: © 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. To associate specimens identified by molecular characters to other biological knowledge, we need reference sequences annotated by Linnaean taxonomy. In this study, we (1) report the creation of a comprehensive reference library of DNA barcodes for the arthropods of an entire country (Finland), (2) publish this library, and (3) deliver a new identification tool for insects and spiders, as based on this resource. The reference library contains mtDNA COI barcodes for 11,275 (43%) of 26,437 arthropod species known from Finland, including 10,811 (45%) of 23,956 insect species. To quantify the improvement in identification accuracy enabled by the current reference library, we ran 1000 Finnish insect and spider species through the Barcode of Life Data system (BOLD) identification engine. Of these, 91% were correctly assigned to a unique species when compared to the new reference library alone, 85% were correctly identified when compared to BOLD with the new material included, and 75% with the new material excluded. To capitalize on this resource, we used the new reference material to train a probabilistic taxonomic assignment tool, FinPROTAX, scoring high success. For the full-length barcode region, the accuracy of taxonomic assignments at the level of classes, orders, families, subfamilies, tribes, genera, and species reached 99.9%, 99.9%, 99.8%, 99.7%, 99.4%, 96.8%, and 88.5%, respectively. The FinBOL arthropod reference library and FinPROTAX are available through the Finnish Biodiversity Information Facility (www.laji.fi) at https://laji.fi/en/theme/protax. Overall, the FinBOL investment represents a massive capacity-transfer from the taxonomic community of Finland to all sectors of society.
- Subjects :
- 0106 biological sciences
Resource (biology)
DNA barcodes
species identification
Biodiversity
reference library
arthropods
Biology
Barcode
Probabilistic taxonomic assignment
010603 evolutionary biology
01 natural sciences
law.invention
Coi
COI
03 medical and health sciences
symbols.namesake
Protax
law
probabilistic taxonomic assignment
niveljalkaiset
Genetics
Animals
DNA Barcoding, Taxonomic
Species identification
Arthropods
Finland
Ecology, Evolution, Behavior and Systematics
Gene Library
030304 developmental biology
0303 health sciences
DNA-analyysi
PROTAX
systematiikka (biologia)
Linnaean taxonomy
Reference library
Geography
Evolutionary biology
Dna barcodes
1181 Ecology, evolutionary biology
symbols
identification
lajinmääritys
Identification (biology)
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Molecular Ecology Resources
- Accession number :
- edsair.doi.dedup.....c611a324c805fff85f3b9c11177a149f