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Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth. Nitrite detection was based on the principle of the Griess reaction, and a linear detection range of 0–1.6 mg/dL (R2 = 0.989) was obtained. Scanning electron microscopy (SEM) analysis demonstrated that the bacteria were able to grow and formed a cluster on the cellulose fibres within 2 hours. The enzyme β-glucuronidase, which is specifically produced by Escherichia coli, was able to convert the pre-immobilized 5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colourless substrate, generating a blue colour. Under optimum conditions, the proposed device allowed bacterial concentrations in the range of 104–107 colony forming units (CFU)/mL to be quantified within 6 hours. Moreover, the use of this device enables the identification of E. coli pathogens with selectivity in real urine samples. In conclusion, the PAD developed in this study for UTI screening provides a rapid, cost-effective diagnostic method for use in remote areas.
- Subjects :
- Paper
0301 basic medicine
lcsh:Medicine
Bacterial growth
medicine.disease_cause
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Griess test
Escherichia coli
medicine
Nitrite
Cellulose
lcsh:Science
Escherichia coli Infections
Nitrites
Multidisciplinary
Chromatography
Filter paper
biology
lcsh:R
Substrate (chemistry)
biology.organism_classification
030104 developmental biology
chemistry
Point-of-Care Testing
Microscopy, Electron, Scanning
lcsh:Q
Colorimetry
030217 neurology & neurosurgery
Bacteria
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....c6041a59ca017b9a8b672a0d969d5aa7