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Temporal expression of adhesion factors and activity of global regulators during establishment of Staphylococcus aureus nasal colonization
- Source :
- The Journal of infectious diseases. 201(9)
- Publication Year :
- 2010
-
Abstract
- The human pathogen Staphylococcus aureus successfully colonizes its primary reservoir, the nasal cavity, most likely by regulatory adaptation to the nose environment. Cotton rats represent an excellent model for the study of bacterial gene expression in the initial phases of colonization. To gain insight into the expression profile necessary for the establishment of colonization, we performed direct transcript analysis by quantitative real-time reverse-transcription polymerase chain reaction on cotton rat noses removed from euthanized animals on days 1, 4, or 10 after instillation of 2 human S. aureus nose isolates. Global virulence regulators (agr, sae) were not active in this early phase, but the essential 2-component regulatory system WalKR seems to play an important role. Accordingly, an elevated expression of walKR target genes (sak, sceD) could be detected. In agreement with previous studies that demonstrated the essential role played by wall teichoic acid (WTA) polymers in nasal colonization, we detected a strongly increased expression of WTA-biosynthetic genes. The expression profile switched to production of the adhesive proteins ClfB and IsdA at later stages of the colonization process. These data underscore the temporal differences in the roles of WTA and surface proteins in nasal colonization, and they provide the first evidence for a regulation of WTA biosynthesis in vivo.
- Subjects :
- Staphylococcus aureus
Virulence
Biology
medicine.disease_cause
Microbiology
Bacterial Proteins
Gene expression
medicine
Immunology and Allergy
Animals
Humans
Colonization
Cotton rat
Sigmodontinae
Adhesins, Bacterial
Gene
Regulation of gene expression
Antigens, Bacterial
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Bacterial
Staphylococcal Infections
biology.organism_classification
Bacterial adhesin
Disease Models, Animal
Nasal Mucosa
RNA, Bacterial
Infectious Diseases
Genes, Bacterial
Trans-Activators
Subjects
Details
- ISSN :
- 15376613
- Volume :
- 201
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- The Journal of infectious diseases
- Accession number :
- edsair.doi.dedup.....c6008ebcb419621c35ce59af0c97f686